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ABT257 Anti-Pro-Collagen Type I, A1/COL1A1

Anti-Pro-Collagen Type I Antibody is an antibody against Pro-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.

Anti-Pro-Collagen Type I, A1/COL1A1: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: COL1A1 UniProt Number: P02452
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ABT257
100 µL  
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      Tabella delle specifiche principali

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, FC, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT257
      DescriptionAnti-Pro-Collagen Type I, A1/COL1A1
      Alternate Names
      • Pro-collagen alpha-1(I) chain
      • Pro-Alpha-1 type I collagen
      Background InformationCollagen alpha-1(I) chain (UniProt P02452; also known as Alpha-1 type I collagen) is encoded by the COL1A1 gene (Gene ID 1277) in human. Collagen is the major component of the extracellular matrix (ECM) and form the fibrils of tendons, ligaments, and bones. Type I collagen consists of two alpha I chains and one alpha 2 chain. Alpha-1 type I collagen is initially produced as an 1464-amino acid prepro-form with a signal peptide sequence (a.a. 1-22) and two propeptide sequences (a.a. 23-161 and a.a. 1219 –1464), the removal of which yields the mature alpha-1(I) chain. The mature alpha-1(I) chain is composed mostly of a large triple-helical region (a.a. 179-1192) sandwiched between two nonhelical segments known as the N-terminal telopeptide (a.a. 162-178; numbering based on the prepro-form) and the C-terminal telopeptide (a.a. 1193-1218; numbering based on the prepro-form). Collagen can be extracted from tissue via either enzymatic or non-enzymatic means. Collagen extracted using the proteolytic enzyme pepsin corresponds to the large triple-helical region, referred to as atelocollagen because both the N- and C-terminal telopeptides have been cleaved off by pepsin. On the other hand, collagen preparations obtained with non-enzymatic means (e.g. by acid extraction) have the intact telopeptides at both ends.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Pro-Collagen Type I Antibody is an antibody against Pro-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected Pro-Collagen Type I, A1/COL1A1 in mouse lung and skin tissue lysate.
      Flow Cytometry Analysis: A representative lot detected Pro-Collagen Type I, A1/COL1A1 in mouse lung tissue.
      Immunohistochemistry Analysis: A representative lot detected Pro-Collagen Type I, A1/COL1A1 in mouse lung tissue.
      Biological Information
      ImmunogenLinear peptide corresponding to sequence derived from human Pro-Collagen Type I, A1/COL1A1 C-terminal propeptide region.
      EpitopeC-terminal propeptide region
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityCat. No. ABT257 Anti-Pro-Collagen Type I, A1/COL1A1 was raised against a sequence derived from the C-terminal propeptide (not the N-terminal propeptide) region. This polyclonal antibody will detect prepro- and pro-, but not mature collagen alpha-1(I) chain.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with a broad range of species based on high sequence homology, including mammalian, avian, fish, and amphibian species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • COL1A1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~140 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse skin tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Pro-Collagen Type I, A1/COL1A1 in 10 µg of mouse skin tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      ABT257 04055977136678

      Documentation

      Anti-Pro-Collagen Type I, A1/COL1A1 Certificati d'Analisi

      TitoloNumero di lotto
      Anti-Pro-Collagen Type I, A1/COL1A1 2902699
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3185049 3185049
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3201707 3201707
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3225879 3225879
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3268885 3268885
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3275493 3275493
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3389910 3389910
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3596724 3596724
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3729946 3729946
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3777980 3777980
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      Informazioni tecniche

      Titolo
      White Paper: Further considerations of antibody validation and usage.

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      Categorie

      Life Science Research > Antibodies and Assays > Primary Antibodies