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MABF2205-25UG Anti-TLR3 Antibody, clone PaT3

MABF2205-25UG
25 μg  
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      Replacement Information
      Description
      Catalogue NumberMABF2205-25UG
      DescriptionAnti-TLR3 Antibody, clone PaT3
      Alternate Names
      • Toll-like receptor 3
      • CD283
      Background InformationToll-like receptor 3 (UniProt: Q99MB1; also known as CD283, TLR3) is encoded by the Tlr3 gene (Gene ID: 142980) in murine species. Toll-like Receptors (TLRs) are transmembrane proteins that are expressed on various immune cells. They contain an N-terminal extracellular and a C-terminal cytoplasmic domain. The N-terminal region contains leucine-rich repeats (LRR) that recognize specific pathogen components. Although the ligand recognition mechanisms of the TLRs vary significantly, all TLR dimers exhibit similar overall arrangement. At least 13 different members of TLR family have been identified that detect different pathogen associated molecular patterns (PAMPs). TLR3 is a single-pass type I membrane glycoprotein that is highly expressed in lung tissue. It is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to produce the mature form that contains a long extracellular domain (aa 26-705), a transmembrane domain (aa 706-726), and cytoplasmic domain (aa 727-925). It exists in monomeric or dimeric form and its dimerization is triggered by ligand binding. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA (dsRNA), a sign of viral infection. It contains 22 LRR repeats and its ds-RNA binding is mediated by LRR 1-3 and 17,18. TLR3 acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response. Clone Pat3 is specific for murine TLR3 and does not react with TLR7, 8, or 9. However, it reacts with both the cleaved and uncleaved forms of TLR3. (Ref.: Murakami, Y., et al. (2014). J. Immunol. 193 (10); 5208-5217).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-TLR3, clone PaT3, Cat. No. MABF2205, is a mouse monoclonal antibody that detects Toll-like receptor 3 and is tested for use in Flow Cytometry and Immunoprecipitation.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated TLR3 in Immunoprecipitation applications (Murakami, Y., et. al. (2014). J Immunol. 193(10):5208-17).

      Flow Cytometry Analysis: A representative lot detected TLR3 in Flow Cytometry applications (Pelka, K., et. al. (2018). Immunity. 48(5):911-922; Murakami, Y., et. al. (2014). J Immunol. 193(10):5208-17).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenBa/F3 cells expressing Flag-6×His-conjugated mouse TLR3. Tlr3-/- mice were immunized.
      EpitopeUnknown
      ClonePaT3
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone PaT3 is a mouse monoclonal antibody that specifically detects murine Toll-like receptor 3.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tlr3
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight103.67 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in J774.2 cells.

      Flow Cytometry Analysis: 2 µg of this antibody detected TLR3 in one million J774.2 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MABF2205-25UG 04061842592135

      Documentation

      Anti-TLR3 Antibody, clone PaT3 MSDS

      Titolo

      Scheda di sicurezza (MSDS) 

      Anti-TLR3 Antibody, clone PaT3 Certificati d'Analisi

      TitoloNumero di lotto
      Anti-TLR3, clone PaT3 - Q3509826 Q3509826

      Riferimenti bibliografici

      Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
      The Chaperone UNC93B1 Regulates Toll-like Receptor Stability Independently of Endosomal TLR Transport
      Karin Pelka 1 , Damien Bertheloot 2 , Elisa Reimer 3 , Kshiti Phulphagar 4 , Susanne V Schmidt 5 , Anette Christ 6 , Rainer Stahl 5 , Nicki Watson 7 , Kensuke Miyake 8 , Nir Hacohen 9 , Albert Haas 10 , Melanie M Brinkmann 3 , Ann Marshak-Rothstein 11 , Felix Meissner 12 , Eicke Latz
      Immunity  48(5)  911-922  2018

      Mostra il sommario
      29768176 29768176
      Roles of the cleaved N-terminal TLR3 fragment and cell surface TLR3 in double-stranded RNA sensing
      Yusuke Murakami 1 , Ryutaro Fukui 1 , Yuji Motoi 1 , Atsuo Kanno 2 , Takuma Shibata 1 , Natsuko Tanimura 1 , Shin-ichiroh Saitoh 1 , Kensuke Miyake
      J Immunol  193(10)  5208-17  2014

      Mostra il sommario
      25305318 25305318