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MAB5430-C Anti-Tau Antibody, Cleaved (D421), clone Tau-C3, Ascites Free

Anti-Tau Antibody, Cleaved (D421), clone Tau-C3, Ascites Free is an antibody against Tau for use in Immunohistochemistry (Paraffin), Immunofluorescence, Western Blotting, Immunocytochemistry.

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Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: MAPT, MAPTL, MTBT1, TAU UniProt Number: P10636
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MAB5430-C
100 μg  
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      Tabella delle specifiche principali

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIH(P), IF, WB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB5430-C
      DescriptionAnti-Tau Antibody, Cleaved (D421), clone Tau-C3, Ascites Free
      Alternate Names
      • Microtubule-associated protein tau
      • Tau, Cleaved (D421)
      • Neurofibrillary tangle protein
      • Paired helical filament-tau
      • PHF-tau
        Microtubule-associated protein tau, Asp421-cleaved N-terminal fragment
      • Neurofibrillary tangle protein, Asp421-cleaved N-terminal fragment
      • Paired helical filament-tau, Asp421-cleaved N-terminal fragment
      • PHF-tau, Asp421-cleaved N-terminal fragment
      Background InformationMicrotubule-associated protein tau (UniProt P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as TAU, MAPT1, MTBTL) gene (Gene ID 4137) in human. Extracellular plaque deposits composed of amyloid-β and intracellular neurofibrillary tangles (NFTs) composed of truncated and hyperphosphorylated tau are two neuropathological hallmarks of Alzheimer's disease (AD). Tau pathology (tauopathy) can cause toxicity when the brain is devoid of amyloid plaques, and tangle pathology correlates better with clinical dementia than amyloid pathology. Abnormal processing of tau by hyperphosphorylation and proteolytic truncation contribute to the toxicity of tau. Tau is known to be cleaved by various proteases, including caspases, calpains, cathepsins, thrombin, and puromycin-sensitive aminopeptidase, as well as the lysosomal cysteine proteinase asparagine endopeptidase (AEP). MAPK, GSK-3, and Cdk-5 are three known kinases that target various tau phosphorylation sites. Tau also undergoes other types of posttranslational modifications, including glycosylation, ubiquitination, glycation, polyamination, nitration, and lysine methylation, which are believed to be important for its non-pathological functions, including polymerization and stabilization of microtubules.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Tau Antibody, Cleaved (D421), clone Tau-C3, Ascites Free is an antibody against Tau for use in Immunohistochemistry (Paraffin), Immunofluorescence, Western Blotting, Immunocytochemistry.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunofluorescence
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunofluorescence Analysis: A representative lot detected truncated Tau cleaved at D421 in the frontal cortex and hippocampus sections from rTg4510 mice expressing human P301L mutant Tau transgene (Ramalho, R.M., et al. (2008). Mol. Med. 14(5-6):309-317).
      Immunofluorescence Analysis: A representative lots detected pathological tangles containing truncated Tau cleaved at D421 by fluorescent immunohistochemistry staining of Alzheimer's diseased (AD) human brain sections (Guillozet-Bongaarts, A.L., et al. (2005). Neurobiol. Aging. 26(7):1015-1022; Horowitz, P.M., et al. (2004). J. Neurosci. 24(36):7895-7902).
      Immunohistochemistry Analysis: A representative lot detected an increase of truncated Tau cleaved at D421 in the brain section from rTg4510 mice expressing human P301L mutant Tau transgene when compared with sections from non-transgenic mice (Ramalho, R.M., et al. (2008). Mol. Med. 14(5-6):309-317).
      Immunohistochemistry Analysis: A representative lot immunostained neurofibrillary tangles (NFTs) and neuritic plaques (NPs) containing truncated Tau cleaved at D421 in frozen hippocampus sections from Alzheimer's diseased (AD) human brains (Gamblin, T.C., et al. (2003). Proc. Natl. Acad. Sci. U. S. A. 100(17):10032-10037).
      Western Blotting Analysis: A representative lot detected truncated Tau cleaved at D421 in frontal cortex and hippocampus tissue extracts from non-transgenic and rTg4510 transgenic mice with human P301L mutant Tau transgene (Ramalho, R.M., et al. (2008). Mol. Med. 14(5-6):309-317).
      Western Blotting Analysis: A representative lots detected recombinantt Tau 1-421 fragment, but not 1-391 or 1-429 fragment or full-length Tau. Clone TauC3 detected tau fragment generated by caspase-mediated D421 cleavage of full-length recombinant Tau (Guillozet-Bongaarts, A.L., et al. (2005). Neurobiol. Aging. 26(7):1015-1022; Park, S.Y., and Ferreira, A. (2005). J. Neurosci. 25(22):5365-5375; Gamblin, T.C., et al. (2003). Proc. Natl. Acad. Sci. U. S. A. 100(17):10032-10037).
      Immunocytochemistry Analysis: A representative lot detected a time-dependent increase of intracellular truncated Tau cleaved at D421 by fluorescent immunocytochemistry staining of paraformaldehyde-fixed primary embryonic rat cortical neurons subjected to fibrilar Aβ (1–42) treament (Gamblin, T.C., et al. (2003). Proc. Natl. Acad. Sci. U. S. A. 100(17):10032-10037).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a human Tau sequence with Asp421 at the C-terminus.
      EpitopeC-terminus.
      CloneTau-C3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone Tau-C3 (TauC3) specifically recognizes caspase-cleaved Tau fragments with Asp421/D421 as the C-termus. Does not react with full-length Tau or Tau fragments with other cleaved C-termi. Uniprot P10636 lists 9 human tau isoforms produced by alternative splicing. The Asp421/D421 designation was based on D421 of isoform 8 (Tau-F, Tau-4), which is equivalent to D738 of isoform 1 (PNS-tau), D332 of isoform 2 (Fetal-tau), D296 of isoform 3 (Tau-A), D361 of isoform 4 (Tau-B), D390 of isoform 5 (Tau-C, Tau-3), D363 of isoform 6 (Tau-D), D392 of isoform 7 (Tau-E), and D756 of isoform 9 (Tau-G).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MAPT
      • MAPTL
      • MTBT1
      • TAU
      Purification MethodProtein G Purified
      UniProt Number
      Molecular WeightVariable, depending on the Tau isoforms where the fragments are derived from and whether the fragements were cleaved at the N-terminus by other proteases.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in Alzheimer's diseased (AD) human brain tissue .

      Immunohistochemistry Analysis: An 1:250 dilution of this antibody detected Asp421-cleaved Tau in neurofibrillary tangles (NFTs) of Alzheimer's diseased (AD) human brain tissue .
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MAB5430-C 04055977330199

      Documentation

      Anti-Tau Antibody, Cleaved (D421), clone Tau-C3, Ascites Free Certificati d'Analisi

      TitoloNumero di lotto
      Anti-Tau, Cleaved (D421), -Q2659002 Q2659002
      Anti-Tau, Cleaved (D421), clone Tau-C3, Ascites Free - 3378586 3378586
      Anti-Tau, Cleaved (D421), clone Tau-C3, Ascites Free - 3488160 3488160
      Anti-Tau, Cleaved (D421), clone Tau-C3, Ascites Free - 3507850 3507850
      Anti-Tau, Cleaved (D421), clone Tau-C3, Ascites Free - 3739685 3739685
      Anti-Tau, Cleaved (D421), clone Tau-C3, Ascites Free - 3892575 3892575
      Anti-Tau, Cleaved (D421), clone Tau-C3, Ascites Free - 3940937 3940937
      Anti-Tau, Cleaved (D421), clone Tau-C3, Ascites Free - 4084422 4084422
      Anti-Tau, Cleaved (D421), clone Tau-C3, Ascites Free - 4313581 4313581
      Anti-Tau, Cleaved (D421), clone Tau-C3, Ascites Free Monoclonal Antibody 2912580