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MABS1935-100UG Anti-α-galactosidase A Antibody, clone 6G8

Anti-a-galactosidase A, clone 6G8, Cat. No. MABS1935, is a mouse monoclonal antibody that detects -a-galactosidase A and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

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MABS1935-100UG
100 μg  
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      Replacement Information
      Description
      Catalogue NumberMABS1935-100UG
      DescriptionAnti-α-galactosidase A Antibody, clone 6G8
      Alternate Names
      • Alpha-D-galactosidase A
      • Alpha-D-galactoside galactohydrolase
      • Galactosylgalactosylglucosylceramidas GLA
      • Melibiase
      • Agalsidase
      Background Informationa-Galactosidase A (UniProt: P06280; EC:3.2.1.22, Alpha-D-galactosidase A, Alpha-D-galactoside galactohydrolase, Galactosylgalactosylglucosylceramidas GLA, Melibiase, Agalsidase) is encoded by the GLA gene (Gene ID: 2717) in human. a-galactosidase A is a homodimeric, lysosomal enzyme that catalyzes the hydrolysis of terminal alpha-galactosyl moieties from glycolipids and glycoproteins and participates to their degradation in the lysosome. It is synthesized with a signal peptide (aa 1-31), which is subsequently cleaved off to generate the mature form that is heavily glycosylated with asparagine-linked high mannose groups. Mutations in GLA gene have been linked to Fabry disease that is characterized by glycolipid accumulation in various tissues and leads to other complication such as renal failure and cardiac and cerebral complications.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-a-galactosidase A, clone 6G8, Cat. No. MABS1935, is a mouse monoclonal antibody that detects -a-galactosidase A and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blot Using KO Lysates
      • Immunohistochemistry
      Application NotesImmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected a-Galactosidase A in HeLa cells.

      Immunohistochemistry Applications: A representative lot detected a-Galactosidase A in Immunohistochemistry applications (Tian, W., et. al. (2019). Nat Commun. 10(1):1785).

      Immunocytochemistry Analysis: A representative lot detected a-Galactosidase A in acetone fixed HEK293 cells either HEK293 overexpressing GLA (A), HEK293 wild type (WT; B), or HEK293 cells with KO of GLA (C) (Courtesy of Asst. Prof. Zhang Yang, Ph.D., and Assoc. Prof. Ulla Mandel, Ph.D., Copenhagen Center for Glycomics, University of Copenhagen, Denmark).

      Western Blotting Analysis: A representative lot detected a-Galactosidase A in lysates from HEK293 cells overexpressing GLA, HEK293 (WT), but not in HEK293 cells with GLA knockout (Courtesy of Asst. Prof. Zhang Yang, Ph.D., and Assoc. Prof. Ulla Mandel, Ph.D., Copenhagen Center for Glycomics, University of Copenhagen, Denmark).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRecombinant full-length human a-Galactosidase A (without the signal peptide).
      EpitopeUnknown
      Clone6G8
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 6G8 is a mouse monoclonal antibody that specifically detects human a-Galactosidase A.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GLA
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight48.77 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in Acetone fixed HEK 293 cells..

      Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected a-Galactosidase A in acetone fixed HEK 293 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MABS1935-100UG 04065266020816