Millipore Sigma Vibrant Logo

HSP1MAG-63K MILLIPLEX MAP Human Sepsis Magnetic Bead Panel 1 - Immune Response Multiplex Assay

Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple sepsis biomarkers in human serum, plasma and cell culture samples.

MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology
View Products on Sigmaaldrich.com
HSP1MAG-63K
Progetti il Suo kit e calcoli il prezzo

Panoramica

Replacement Information

Tabella delle specifiche principali

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
MIF PAI-1 (Total) sFas sFasL sICAM-1 sVCAM-1 HMplexLuminex xMAP
Description
Catalogue NumberHSP1MAG-63K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Sepsis Magnetic Bead Panel 1 - Immune Response Multiplex Assay
Background InformationSepsis or SIRS (systemic inflammatory response syndrome) results from infection, whether bacterial, viral, fungal or parasitic. According to the CDC, sepsis is the second leading cause of death in non-coronary ICU patients in the U.S. The pathogenesis of sepsis is very complex. Both an innate and acquired immune response to infection interrupts homeostasis with a shift of cytokine expression that increases immunosuppression. In addition migration of the leukocytes in the inflamed tissues secretes a tissue factor leading to the formation of thrombin, also triggering the release of chemokines and adhesion molecules through endothelial cells. Severe sepsis occurs when hypotension or hypoperfusion to one or more organs leads to organ dysfunction, which, in turn, can cause septic shock, multiple organ dysfunction syndrome (MODS) and death.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 15
  • Intra-assay: < 5
Panel TypeMAGNETIC Immune Response
Quality LevelMQ200
Applications
ApplicationInflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple sepsis biomarkers in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This assay requires 25 µL of diluted serum or plasma (1:10)
• This assay can also be used with tissue culture samples or homogenates
• Overnight incubation is recommended
Biological Information
Species Reactivity
  • Human
Analytes Available
  • MIF
  • PAI-1 (Total)
  • sFas
  • sFasL
  • sICAM-1
  • sVCAM-1
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • MIF 114%
  • PAI-1 (total) 98%
  • sFas 98%
  • sFasL 106%
  • sICAM-1 104%
  • sVCAM-1 111%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for individual sensitivities.
Standard Curve Range
  • 61–250,000 pg/mL for sVCAM-1 and sICAM-1
  • 12–50,000 pg/mL for sFas, sFasL, and PAI-1
  • Refer to QC analysis sheet for exact concentration for MIF
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
HSP1MAG-63K 04053252987632