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SCYT-91K-PXBK14 MILLIPLEX® Ovine Cytokine/Chemokine Panel 1 Premixed 14-plex Space Saver (Bulk) Packaging - Immunology Multiplex Assay

The Ovine Cytokine/Chemokine bead-based multiplex cytokine panel, using the Luminex xMAP technology, enables the simultaneous analysis of 14 cytokine and chemokine biomarkers in ovine serum, plasma and cell culture samples.

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Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms, Inflammation, Infectious Diseases, Cytokines & Cytokine Receptors, Oncology, Obesity, Metabolic Disorders
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SCYT-91K-PXBK14
96-well plate  96 well plate
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      Tabella delle specifiche principali

      Analytes AvailableSpecies ReactivityKey ApplicationsMultiplexing Ordering RequirementsDetection Methods
      IFN-γ IL-1α IL-1β IL-4 IL-6 IL-10 IL-8 IL-17A MIP-1α IL-36RA IP-10 MIP-1β TNFα VEGF-A ShMplexNote: The protocol recommends a 1:2 dilution of serum and plasma samples, except when assaying IL-8 (CXCL8) in serum samples for which an up to 1:8 dilution of sample may be required.Luminex xMAP
      Description
      Catalogue NumberSCYT-91K-PXBK14
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX® Ovine Cytokine/Chemokine Panel 1 Premixed 14-plex Space Saver (Bulk) Packaging - Immunology Multiplex Assay
      OverviewMILLIPLEX® Ovine Cytokine/Chemokine Panel 1 may be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, and tissue culture supernatant samples: IFNγ, IL-1α, IL-1β, IL-4, IL-6, IL-8 (CXCL8), IL-10, IL-17A, IL-36RA (IL-1F5), IP-10 (CXCL10), MIP-1α (CCL3), MIP-1β (CCL4), TNFα, VEGF-A. Note: The protocol recommends a 1:2 dilution of serum and plasma samples, except when assaying IL-8 (CXCL8) in serum samples for which an up to 1:8 dilution of sample may be required.

      MILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). In addition each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

      Each MILLIPLEX® panel and kit includes:
      • Quality controls (QCs) to qualify assay performance
      • Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
      • Optimized serum matrix to mimic native analyte environment in serum and plasma samples
      • Detection antibody cocktails designed to yield consistent analyte profiles within panel

      The MILLIPLEX® Ovine Cytokine/Chemokine Panel 1 thus enables you to focus on the therapeutic potential of cytokines and the modulation of cytokine expression.
      Background InformationInflammation and immune cell response are key to maintaining homeostasis. Inflammation plays a central role in the etiology of many immunologically related diseases, such as infectious disease, osteoarthritis and respiratory disease, as well as neurologic, metabolic and cardiovascular disease and even cancer. However, until now the ability to measure levels of cytokines and chemokines in sheep samples has been limited primarily to ELISAs. Researchers now have the ability to measure multiple inflammation biomarkers simultaneously using our MILLIPLEX® Ovine Cytokine/Chemokine Panel 1, based on the Luminex® xMAP® technology.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Precision, %
      • Intra-assay: <10%
      • Inter-assay: <20%
      Multiplex Ordering RequirementsNote: The protocol recommends a 1:2 dilution of serum and plasma samples, except when assaying IL-8 (CXCL8) in serum samples for which an up to 1:8 dilution of sample may be required.
      Panel TypeMAGNETIC Cytokines/Chemokines
      Applications
      ApplicationThe Ovine Cytokine/Chemokine bead-based multiplex cytokine panel, using the Luminex xMAP technology, enables the simultaneous analysis of 14 cytokine and chemokine biomarkers in ovine serum, plasma and cell culture samples.
      Key Applications
      • Multiplexing
      Application Notes• This is an overnight incubation assay.
      • The protocol recommends a 1:2 dilution of serum and plasma samples, except when assaying IL-8 (CXCL8) in serum samples for which an up to 1:8 dilution of sample may be required.
      • This kit may be used for the analysis of any or all of the Ovine Cytokine/Chemokine Panel 1 analytes in ovine tissue/cell lysate and culture supernatant samples and serum or plasma samples.
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Sheep
      Analytes Available
      • IFN-γ
      • IL-1α
      • IL-1β
      • IL-4
      • IL-6
      • IL-10
      • IL-8
      • IL-17A
      • MIP-1α
      • IL-36RA
      • IP-10
      • MIP-1β
      • TNFα
      • VEGF-A
      Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Accuracy
      • 83% - 115%
      Physicochemical Information
      Sensitivity
      • Refer to kit protocol for sensitivities for individual cytokines and chemokines.
      Standard Curve Range
      • IL-1α: 0.19 - 3,000 pg/mL
      • IFNγ: 0.32 - 5,000 pg/mL
      • IL-36RA: 0.35 - 5,500 pg/mL
      • IL-8, IP-10, VEGF-A: 0.77 - 12,000 pg/mL
      • IL-10, IL-17A: 1.6 - 25,000 pg/mL
      • MIP-1β: 3.8 - 60,000 g/mL
      • IL-4: 6.4 - 100,000 pg/mL
      • IL-6, MIP-1α: 7.7 - 120,000 pg/mL
      • IL-1β: 12.8 - 200,000 pg/mL
      • TNFα: 48 - 750,000 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96 well plate
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      SCYT-91K-PXBK14 04061842306916