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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Opzione salva-spazio I Clienti che ordinano diversi kit possono scegliere di ridurre lo spazio necessario per lo stoccaggio, rinunciando al confezionamento del kit e ricevendo i vari reagenti per il saggio multiplex in buste di plastica.
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566807
Sigma-AldrichPhosphoDetect™ Anti-Shc/p66 (pSer³⁶) Mouse mAb (6E10)
This PhosphoDetect™ Anti-Shc/p66 (pSer³⁶) Mouse mAb (6E10) is validated for use in ELISA, Immunoblotting for the detection of Shc/p66 (pSer³⁶).
More>>This PhosphoDetect™ Anti-Shc/p66 (pSer³⁶) Mouse mAb (6E10) is validated for use in ELISA, Immunoblotting for the detection of Shc/p66 (pSer³⁶). Less<<
MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
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Description
Overview
Recognizes the ~66 kDa form of Shc phosphorylated at Ser36. Does not cross-react with the non-phosphorylated form of Shc/p66 or with unrelated phosphorylation sites.
Catalogue Number
566807
Brand Family
Calbiochem®
Application Data
Detection of human Shc/p66, phosphorylated on Ser36, by immunoprecipitation. Samples: Timecourse of HeLa cells stimulated with EGF. Primary antibody: PhosphoDetect™ Anti-Shc/p66 (pSer36) Mouse mAb (6E10) (Cat. No. 566807) (1 µg/ml). Detection: chemiluminescence.
References
References
Foschi, M., et al. 2001. J. Biol. Chem.276, 26640. Gu, H., et al. 2000. Mol. Cell. Biol.20, 7109. Migliaccio, E., et al. 1997. EMBO J.16, 706. Okada, S., et al. 1997. J. Biol. Chem.272, 28042. Pelicci, G., et al.1992. Cell70, 93.
Product Information
Form
Lyophilized
Formulation
100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 125 µl control protein lyophilized from 2X PBS.
For immunoblotting using the p66/Shc positive control, use 15 µl per lane for colorimetric or chemiluminescent detection. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁶ phosphorylation site of human Shc/p66
Clone
6E10
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute the antibody with 1 ml dH₂O for at least 15 min at room temperature until completely dissolved. Following reconstitution, aliquot and freeze (-20°C). Reconstitute the positive control with 125 µl H₂O until completely dissolved. After complete solubilization, add 125 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo
GTIN
566807-1SET
04055977267082
Documentation
PhosphoDetect™ Anti-Shc/p66 (pSer³⁶) Mouse mAb (6E10) MSDS
Foschi, M., et al. 2001. J. Biol. Chem.276, 26640. Gu, H., et al. 2000. Mol. Cell. Biol.20, 7109. Migliaccio, E., et al. 1997. EMBO J.16, 706. Okada, S., et al. 1997. J. Biol. Chem.272, 28042. Pelicci, G., et al.1992. Cell70, 93.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
15-August-2007 RFH
Application
ELISA (0.05 µg/ml) Immunoblotting (0.1-1 µg/ml)
Application Data
Detection of human Shc/p66, phosphorylated on Ser36, by immunoprecipitation. Samples: Timecourse of HeLa cells stimulated with EGF. Primary antibody: PhosphoDetect™ Anti-Shc/p66 (pSer36) Mouse mAb (6E10) (Cat. No. 566807) (1 µg/ml). Detection: chemiluminescence.
Description
Immunoaffinity purified mouse monoclonal antibody. Recognizes the Shc/p66 protein phosphorylated on Ser36. Supplied with a positive control consisting of Shc/p66 from EGF-stimulated HepG2 cells immunoprecipitated using anti-Shc non-phospho-specific antibody.
Background
Mammalian cells can express three alternatively spliced isoforms of the shc adaptor protein: Shc/p46, Shc/p52 and Shc/p66. Shc/p46 and Shc/p52 are found in every cell type, whereas Shc/p66 expression varies in different tissues. Shc proteins are phosphorylated by receptor tyrosine kinases and interact with phosphotyrosine residues on these receptors via the shc PTB and/or SH2 domains. The major tyrosine phosphorylation sites are tyrosines 239/240 as well as tyrosine 317. The Shc/p66 protein contains an additional N-terminal protein domain that is phosphorylated at serine 36. It is suggested that only the serine-phosphorylated Shc/p66 can interact with GRB2. Moreover, expression of Shc/p66 accelerates the inactivation of MAP kinases after EGF stimulation, whereas Shc/p46 and Shc/p52 enhance MAP kinase activity.
Host
Mouse
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁶ phosphorylation site of human Shc/p66
Clone
6E10
Isotype
IgG₁
Species
human, mouse
Form
Lyophilized
Formulation
100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 125 µl control protein lyophilized from 2X PBS.
Preservative
≤0.1% sodium azide (antibody only)
Comments
For immunoblotting using the p66/Shc positive control, use 15 µl per lane for colorimetric or chemiluminescent detection. Variables associated with assay conditions will dictate the proper working dilution.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute the antibody with 1 ml dH₂O for at least 15 min at room temperature until completely dissolved. Following reconstitution, aliquot and freeze (-20°C). Reconstitute the positive control with 125 µl H₂O until completely dissolved. After complete solubilization, add 125 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Toxicity
Standard Handling
References
Foschi, M., et al. 2001. J. Biol. Chem.276, 26640. Gu, H., et al. 2000. Mol. Cell. Biol.20, 7109. Migliaccio, E., et al. 1997. EMBO J.16, 706. Okada, S., et al. 1997. J. Biol. Chem.272, 28042. Pelicci, G., et al.1992. Cell70, 93.
