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Merck

A2172

Anti-α-Actin-1 (ACTA1) antibody

mouse monoclonal, 5C5

Synonyme(s) :

Mouse Anti-Actin

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00164521

Principaux documents

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Nom du produit

Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse, clone 5C5, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

5C5, monoclonal

contains

15 mM sodium azide

species reactivity

rabbit, carp, rat, sheep, guinea pig, human, snake, bovine, frog

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human skeletal and cardiac muscle
immunohistochemistry (frozen sections): suitable using human skeletal and cardiac muscle
indirect ELISA: suitable
western blot: suitable

isotype

IgM

UniProt accession no.

P68032
P68133

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... ACTA1(58), ACTC1(70)
mouse ... Rwdd2a(69519)
rat ... Acta1(29437), Actc1(29275)

Application

Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse has also been used
  • immunofluorescence
  • double immunostaining
  • double labelling terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) immunofluorescence staining.

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.
Mouse monoclonal anti-actin (alpha-sarcomeric) antibody has been used for western blot, and immunohistochemistry applications.

Biochem/physiol Actions

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-α-Sarcomeric Actin (mouse IgM isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin is one of the most conserved eukaryotic proteins and the isoforms show >90% overall sequence homology. It is expressed in mammals and birds as six isoforms. Four of the six represent differentiation markers of muscle tissues. The other two are found in practically all cells.

Immunogen

rabbit striated muscle actin.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Physical form

The product is provided as ascites fluid containing 0.1% sodium azide as a preservative.

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Classe de stockage

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000521925
0000521925
0000480981
0000480981
0000334310

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Consulter la Bibliothèque de documents

PEDF and PEDF-derived peptide 44mer stimulate cardiac triglyceride degradation via ATGL
Zhang H, et al.
Journal of Translational Medicine, 13(1), 68-68 (2015)
PEDF improves cardiac function in rats subjected to myocardial ischemia/reperfusion injury by inhibiting ROS generation via PEDF-R
Zhao Q, et al.
International Journal of Molecular Medicine, 41(6), 3243-3252 (2018)
Forced myocardin expression enhances the therapeutic effect of human mesenchymal stem cells after transplantation in ischemic mouse hearts
Grauss RW, et al.
Stem Cells, 26(4), 1083-1093 (2008)
Xiao Gao et al.
Scientific reports, 4, 5637-5637 (2014-07-12)
Pigment epithelium-derived factor (PEDF) has many biological activities. But it's not known whether PEDF and its functional peptides could protect against hypoxia-induced cell death and the mechanisms are still unclear. We used cultured H9c2 cells and primary cardiomyocytes to show
Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle
Chaponnier C and Gabbiani G
F1000Research, 5 (2016)
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