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Merck

H3506

Hyaluronidase from bovine testes

Type I-S, lyophilized powder, 400-1000 units/mg solid

Synonyme(s) :

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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About This Item

Numéro CAS:
37326-33-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
MFCD00131351
Numéro CE :
3.2.1.35 (BRENDA, IUBMB)

Nom du produit

Hyaluronidase from bovine testes, Type I-S, lyophilized powder, 400-1000 units/mg solid

type

Type I-S

form

lyophilized powder

specific activity

400-1000 units/mg solid

mol wt

~55 kDa (four subunits of 14 kDa each)

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

UniProt accession no.

Q5E985
Q8SQG8

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

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Catégories apparentées

Biochem/physiol Actions

L′hyaluronidase dégrade l′hyaluronane et elle semble être régulée de façon anormale lors de la progression du cancer.
Ces enzymes coupent de façon aléatoire les liaisons glycosidiques β-N-acétylhexosamine-[1→4] dans l′acide hyaluronique, la chondroïtine et les sulfates de chondroïtine.

Application

L'hyaluronidase de testicules bovins a été utilisée pour la digestion de tissus mammaires, de tissus cardiaques et d'ovocytes pour isoler des cellules.

Ce produit peut être utilisé pour préparer des suspensions de cellules individuelles en vue d'un séquençage.

Other Notes

Pour en savoir plus sur les enzymes pour l′analyse des glucides complexes, rendez-vous sur www.sigma-aldrich.com/enzymeexplorer.
Une unité dégrade 0,75 μg du polysaccharide acide hyaluronique par minute à pH 5,35 et 37 ºC (valeur mesurée par absorbance turbidimétrique [λ : 600 nm] sous forme complexée avec de la BSA après 45 minutes).

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000522169
0000522169
0000522170
0000522170
0000382434

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. How is Hyaluronidase from bovine testes, Product H3506, solubilized?

    It is soluble at 1 mg/ml in 0.02 M phosphate buffer, pH 7 containing 77 mM sodium chloride and 0.01% bovine serum albumin.

  3. How is a solution of Hyaluronidase from bovine testes, Product H3506, stored?

    Prepare fresh solutions directly before use since stock solutions are not stable for storage.

  4. Can Hyaluronidase from bovine testes, Product H3506, be used on humans?

    No. None of our packaged research products are for human use. 

  5. What is the shelf life of Product H3506, Hyaluronidase from bovine testes?

    Product H3506, Hyaluronidase from bovine testes, has a Recommended Retest period of two years (period of time between the Quality Control Release and Recommended Retest dates).  This date assigns the time frame during which the batch is expected to remain within established specifications if stored under Sigma-Aldrich’s defined conditions, which for product H3506 is at -20°C.  This information is provided on the Product's lot specific Certificate of Analysis. 

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Highly efficient gene transfer into adult ventricular myocytes by recombinant adenovirus.
Kirshenbaum LA et al.
The Journal of Clinical Investigation, 92, 381-381 (1993)
Heikki J Nieminen et al.
Scientific reports, 7(1), 3991-3991 (2017-06-24)
Research is ongoing to develop drug therapies to manage osteoarthritis (OA) and articular cartilage (AC) injuries. However, means to deliver drug to localized AC lesions are highly limited and not clinically available. This study investigates the capability of laser ultrasound
Effect of pressure at primary drying of freeze-drying mouse sperm reproduction ability and preservation potential.
Kawase Y et al.
Reproduction (Cambridge, England), 133, 841-841 (2007)
Moshe Sade-Feldman et al.
Nature communications, 8(1), 1136-1136 (2017-10-27)
Treatment with immune checkpoint blockade (CPB) therapies often leads to prolonged responses in patients with metastatic melanoma, but the common mechanisms of primary and acquired resistance to these agents remain incompletely characterized and have yet to be validated in large
A SAGE (serial analysis of gene expression) view of breast tumor progression.
Porter DA et al.
Cancer Research, 61, 5697-5697 (2001)
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