Gel electrophoresis

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Dispositif automatisé d'électrophorèse 2D sur gel Auto2D®

Le dispositif d'électrophorèse 2D Auto2D® automatise entièrement les méthodes d'électrophorèse 2D difficiles et les rend plus rapides, faciles et reproductibles. Repérez les protéines difficiles à localiser à l'aide du système Auto2D® en moins de deux heures avec une reproductibilité élevée.

Système d'eau de laboratoire pure Milli-Q^® IX

De l'eau pure de Type 2 à partir d'eau de ville. Les systèmes Milli-Q® IX sont compacts, offrent 4 débits et 3 tailles de cuve, et permettent de surveiller la qualité de l'eau directement sur l'écran tactile du distributeur.

Présentation de l'électrophorèse SDS-PAGE (séparation des protéines selon leur taille)

Présentation de l'électrophorèse PAGE. Découvrez l'histoire et le protocole de l'électrophorèse SDS-PAGE pour la séparation des protéines en fonction de leur taille sur un gel de polyacrylamide.

Électrophorèse sur gel

Les méthodes d'électrophorèse des protéines sur gel permettent de séparer les protéines en vue d'applications downstream comme le western blotting et la spectrométrie de masse.

Système de coulée mPAGE^®Lux pour les gels SDS-PAGE

Rafraîchissez votre flux de travail de coulée de gel avec des gels SDS-PAGE prêts à l'emploi en seulement 3 minutes. Découvrez des méthodes de coulée de gels plus rapides, plus simples et plus sûres.

Coulage manuel de gels de polyacrylamide pour PAGE et SDS-PAGE à l'aide des kits de coulage de gels Bis-Tris mPAGE® TurboMix

Vue d'ensemble des propriétés chimiques des gels de polyacrylamide et détail des consignes à suivre pour les couler à l'aide des kits de coulage de gels Bis-Tri mPAGE® TurboMix.

Protein Visualization

Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.

DNA / Protein Electrophoresis and Troubleshooting Tables

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

Precipitation Procedures

This page describes protein precipitation as an optional step in sample preparation for 2-D electrophoresis with Cytiva products.

Performing a Purity and Homogeneity Check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

Troubleshooting first-dimension IEF: Ettan™ IPGphor™ 3 Isoelectric Focusing System

Troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from Cytiva.

Preparing for IEF

This protocol shows how to prepare the Immobiline® DryStrip Kit and electrodes strips.

Reproducibility with Biological Buffers

Biological buffers are organic substances that maintain a constant pH over a given range by neutralizing the effects of hydrogen ions.

Formamide Molecular Biology Reagent

Formamide is a reagent that is an ionizing solvent in aqueous buffers. It is widely utilized in biochemistry and molecular biology, particularly in nucleic acids research.

Paper-Bridge Loading

This page shows how to apply higher sample volumes and protein amounts with paper bridges in isoelectric focusing with Cytiva products

Applying Equilibrated Immobiline® DryStrip Gels

This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.

Detection of GST-tagged Proteins with Western Blot

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.

Proteomic analysis of membrane proteins

This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from Cytiva.

Auto2D® 2-D Gel Electrophoresis Device Frequently Asked Questions

Auto2D® 2-D Gel Electrophoresis Device frequently asked questions for 2-D PAGE and additional 2DE protein separation workflow needs.

How to Run an mPAGE™ Protein Gel Using a Bio-Rad Electrophoresis System

Instructions for setting up an mPAGE SDS-PAGE precast protein gel for gel electrophoresis using a Bio-Rad Mini-PROTEAN® electrophoresis system.

Winning Westerns Technique Talk: Perfecting Western Blotting

In this webinar, Alli Lynch, Ph.D. reviews the basics of Western blotting, identifies common troubleshooting issues, and provides tips for Western blotting success.

Precast Agarose Gels for RNA Electrophoresis

Precast Agarose Gels for RNA Electrophoresis are suitable for separating RNA sizes from 0.25 to 10 kb.

Auto2D® Automated 2-D Gel Electrophoresis Device Protocol

The Auto2D® Automated 2-D Gel Electrophoresis Device video and detailed protocol provide reliable methods to prepare your samples and operate the Auto2D® Device.

ReadyBlue Protein Gel Staining Protocols

ReadyBlue™ Protein Gel Stain offers rapid Coomassie® staining for polyacrylamide gels with a simplified protocol.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Two-Dimensional Separation of Protein Samples

This page describes two-dimensional separation of protein samples with Cytiva products.

Troubleshooting 2-D DIGE Results

Troubleshooting table for 2D DIGE results lists problems, causes, and prevention methods for future experiments.

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

Gel Electrophoresis

Protein gel electrophoresis methods are used for protein separation in preparation for downstream applications like Western blotting and mass spectrometry.

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