Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Viruses cause a diverse array of infectious diseases in humans, including the common cold, chicken pox, AIDS and SARS, just to name a few. Understanding how viruses interact with and proliferate within host cells, as well as how viruses evade or co-opt the functioning immune system, is of central importance for developing therapies to fight viral diseases. Using the multiparametric image-based approach enabled by Amnis® imaging flow cytometry, it is possible to identify virally infected cells and measure the impact of that infection on signal transduction cascades, cell morphology and cell death.
Co-Localization of CpGB to Lysosomes and Endosomes in Primary Plasmacytoid Dendritic Cells
Binding, internalization, and colocalization to lysosomes of CpGB by plasmacytoid dendritic cells (pDC) is measured using the ImageStream®. In combination with immunophenotyping, spatial resolution and intensities of lysosomes (green) are correlated with CpGB (red) in pDC (orange). This data highlights several strengths of the ImageStream®: 1) Combination of immunophenotyping (pDC identification via fluorescent intensity staining) with image correlation and internalization analysis, allowing measurement of colocalization and internalization of molecules within specific subsets. 2) Ability to measure these events in small, primary cells with small cytoplasmic compartments. For the complete time course of endosome and lysosome co-localization, see the application note.
HIV-Specific Translocation of NFAT
HIV-specific nuclear localization of NFAT was measured in HIV-experienced T cells from peripheral blood of HIV+ patients. HIVgag peptide specifically stimulates NFAT (green) to move to the nucleus (red) in HIV-tetramer positive cells (orange). The Similarity score correlates DRAQ5 nuclear stain with the NFAT signal. The higher the Similarity score, the more translocation is visualized in the example images from each quadrant.
Viruses cause a diverse array of infectious diseases in humans, including the common cold, chicken pox, AIDS and SARS, just to name a few. Understanding how viruses interact with and proliferate within host cells, as well as how viruses evade or co-opt the functioning immune system, is of central importance for developing therapies to fight viral diseases. Using the multiparametric image-based approach enabled by Amnis® imaging flow cytometry, it is possible to identify virally infected cells and measure the impact of that infection on signal transduction cascades, cell morphology and cell death.
