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AB10021 Anti-Histone H2A.X Antibody,Chicken

Anti-Histone H2A.X Antibody, Chicken is a Rabbit Polyclonal Antibody for detection of Histone H2A.X also known as H2AFX & has been validated in WB.

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: H2AFX, H2A.X UniProt Number: P16104
AB10021
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      ChWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB10021
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Histone H2A.X Antibody,Chicken
      Alternate Names
      • H2AFX
      Background InformationHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. H2A.X, also known as H2AFX, is a variant of Histone H2A that is found in a subset of nucleosomes. H2A.X has a unique C-terminal tail that becomes phosphorylated following double-stranded DNA breaks following irradiation or apoptosis. Phosphorylation of H2A.X on Ser139 is one of the earliest markers for apoptosis and DNA damage.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Chicken heart tissue lysate
      PresentationPurified from rabbit serum by affinity chromatography. Supplied at 1 mg/ml in PBS with 0.1% NaN3.
      Applications
      ApplicationAnti-Histone H2A.X Antibody, Chicken is a Rabbit Polyclonal Antibody for detection of Histone H2A.X also known as H2AFX & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenThe immunogen used was a synthetic peptide TFKSTGVPKEQPGSQ-C, corresponding to a.a. 125-139 of chicken H2A.X, coupled to KLH.
      Epitopea.a. 125-139
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Chicken H2A.X, Mr 15 kDa.
      Species Reactivity
      • Chicken
      Species Reactivity NoteChicken. Does not recognize human H2A.X. Cross-reactivity with other species has not been tested..
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
      Gene Symbol
      • H2AFX
      • H2A.X
      Purification MethodPeptide affinity chromatography
      UniProt Number
      UniProt Summary [FUNCTION] Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
      [SUBUNIT] The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140. These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11A, RAD50, and NBN. Interaction with the MRN complex is mediated at least in part by NBN. Also interacts with DHX9/NDHII when phosphorylated on Ser-140.
      Molecular Weight15 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on lysates from chicken heart tissue
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB10021 04053252676857

      Documentation

      Related Products & Applications

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      Catalogue Number Description  
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      Accessories

      Catalogue Number Description  
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      07-164 Anti-phospho-H2A.X (Ser139) Antibody Show Pricing & Availability
      16-193 Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate Show Pricing & Availability
      16-202A Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate Show Pricing & Availability
      17-327 H2A.X Phosphorylation Assay Kit (Chemiluminescence Detection) Show Pricing & Availability

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies