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MABE132 Anti-MyoD Antibody, clone 5F11

Detect MyoD using this Anti-MyoD Antibody, clone 5F11 validated for use in Western Blotting, ICC.

Anti-MyoD Antibody, clone 5F11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: Myod1, Myod UniProt Number: P10085
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MABE132
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, ICCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE132
      ReplacesMABE514
      DescriptionAnti-MyoD Antibody, clone 5F11
      Alternate Names
      • Myoblast determination protein 1
      Background InformationMyoblast determination protein 1 (MyoD1) is a myogenic transcription factor required for the growth, repair, and regeneration of muscle cells. It induces differentiation of myoblast cells into multinucleated myotubules, activating genes and proteins involved in cell cycle arrest, and differentiation. MyoD1 may also control proliferation and homeostasis during embryogenesis, in the absence of Myf5.
      References
      Product Information
      FormatPurified
      Control
      • C2C12 cell lysate
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect MyoD using this Anti-MyoD Antibody, clone 5F11 validated for use in Western Blotting, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected MyoD in C2C12 cells.

      Western Blot Analysis: A representative lot detected MyoD in C2C12 cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to mouse MyoD.
      Clone5F11
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Myod1
      • Myod
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~45 kDa observed. An uncharacterized band at ~38 kDa may be observed in some cell lysates. The calculated molecular weight is 34 kDa; however, this protein is known to be glycosylated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in C2C12 cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected MyoD in 10 µg of C2C12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE132 04053252440830

      Documentation

      Anti-MyoD Antibody, clone 5F11 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-MyoD Antibody, clone 5F11 Certificates of Analysis

      TitleLot Number
      Anti-MyoD, clone 5F11 - 2370185 2370185
      Anti-MyoD, clone 5F11 - 2291000 2291000
      Anti-MyoD, clone 5F11 - 2291000A 2291000A
      Anti-MyoD, clone 5F11 - 3230534 3230534
      Anti-MyoD, clone 5F11 - 3506605 3506605
      Anti-MyoD, clone 5F11 - 3766698 3766698
      Anti-MyoD, clone 5F11 - 3819978 3819978
      Anti-MyoD, clone 5F11 - 3928034 3928034
      Anti-MyoD, clone 5F11 - 4130129 4130129
      Anti-MyoD, clone 5F11 - 4197602 4197602
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