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PRCYASF-40K MILLIPLEX® Non-Human Primate Cytokine/Chemokine/Growth Factor Panel A SF, China only

Simultaneously analyze up to 48 cytokine, chemokine, and growth factor biomarkers with a bead-based multiplex assay using Luminex® xMAP® technology, in non-human primate cell culture samples.

PRCYASF-40K

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Description
Catalogue NumberPRCYASF-40K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Non-Human Primate Cytokine/Chemokine/Growth Factor Panel A SF, China only
OverviewMILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. Each MILLIPLEX® panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Detection antibody cocktails designed to yield consistent analyte profiles within panel
Background Information“Cytokine” is a general term used for a diverse group of small proteins, peptides or glycoproteins secreted by lymphocytes, monocytes, macrophages and other cells that regulate immune responses, haematopoiesis and lymphocyte development. Cytokines include interleukins, interferons, chemokines and other signaling molecules. Growth factors are extracellular polypeptides that have a positive effect on cell growth and proliferation. They can stimulate growth in a variety of different cell types. Growth factors and cytokines are similar in structure and the way of action. Individual cytokines or growth factors are produced by multiple cell types. This is different from hormones, which tend to be made by specialized glands. Each cytokine or growth factor acts through its own receptor on target cells to generate signaling pathways, and as a consequence to regulate biological processes. Some intracellular signaling components are shared between cytokines and growth factors. Expression of cytokines or growth factors and their receptors is highly regulated. De-regulation may contribute to many diseases such as infectious disease, autoimmune and chronic inflammatory disease, cardiovascular disease, metabolic syndrome, neurological disorders and cancer. Cytokine and growth factor research plays a significant role in achieving a deeper understanding of the immune system and combating related diseases. As the most closely related species to humans, non-human primates are critical models for those studies.

The MILLIPLEX® Non-Human Primate Cytokine/Chemokine/Growth Factor Panel A Magnetic Bead Panel is a 48-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysate and culture supernatant samples: BCA-1, sCD137, sCD40L, Eotaxin, sFASL, FGF-2, Fractalkine, G-CSF, GM-CSF, Granzyme A, Granzyme B, IFNα2, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-15, IL-16, IL-17A, IL-17E, IL-18, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10, I-TAC, MCP-1, MIG, MIP-1α, MIP-1β, MIP-3α, Perforin, RANTES, TGFα, TNFα, TNFβ, VEGF-A.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Multiplex Ordering RequirementsFor questions, contact Technical Service.
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneously analyze up to 48 cytokine, chemokine, and growth factor biomarkers with a bead-based multiplex assay using Luminex® xMAP® technology, in non-human primate cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay.
• This assay requires 25 µL of cell culture supernatant per well.
• This kit may be used for the analysis of any or all of the Non-Human Primate Cytokine/Chemokine/Growth Factor Panel A analytes in human tissue/cell lysate and culture supernatant samples.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Non-human Primate
Analytes Available
  • G-CSF
  • GM-CSF
  • IFN-γ
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-8
  • IL-10
  • IL-15
  • IL-18
  • MCP-1
  • MIP-1α
  • MIP-1β
  • TGF-α
  • TNF-α
  • BCA-1
  • sCD137
  • CD40 Ligand
  • Eotaxin
  • sFasL
  • Fractalkine
  • Granzyme A
  • Granzyme B
  • IFNα2
  • IFN-α
  • IL-1Rα
  • IL-7
  • IL-12(p70)
  • IL-16
  • IL-17A
  • IL-17E/IL-25
  • IL-21
  • IL-22
  • IL-23
  • IL-28A
  • IL-31
  • IL-33
  • IP-10
  • I-TAC
  • MIG
  • MIP-3α
  • Perforin
  • RANTES
  • TNFβ
  • VEGF-A
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel, except G-CSF had ~5% cross-reactivity with IL-1β.
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities for individual cytokines/chemokines
Standard Curve Range
  • sCD40L: 48.83 - 200,000 pg/mL
  • BCA-1, Eotaxin, GM-CSF, IL-1α, IL-2, IL-17A, IL-6, IP-10, I-TAC, MIP-3α, TNFβ: 1.22 - 5,000 pg/mL
  • FGF-2: 61.04 - 250,000 pg/mL
  • sCD137, Granzyme B, IL-1β: 0.98 - 4,000 pg/mL
  • Fractalkine, IL-17E: 24.41 - 100,000 pg/mL
  • IFNγ, IFNα2, IL-10, IL-22, VEGF-A: 2.44 - 10,000 pg/mL
  • IL-16: 36.62 - 150,000 pg/mL
  • sFASL, G-CSF, IL-1RA, IL-21, IL-7, MIG, TNFα: 4.88 - 20,000 pg/mL
  • Granzyme A: 0.61 - 2,500 pg/mL
  • IL-15, MIP-1β: 6.10 - 25,000 pg/mL
  • IL-8: 0.05 - 200 pg/mL
  • IL-23, IL-18: 19.53 - 80,000 pg/mL
  • IL-12(p70), IL-28A, IL-31, IL-4: 12.21 - 50,000 pg/mL
  • IL-33: 14.65 - 60,000 pg/mL
  • IL-5: 2.93 - 12,000 pg/mL
  • MCP-1: 3.05 - 12,500 pg/mL
  • MIP-1α: 0.73 - 3,000
  • RANTES: 0.10 - 400
  • TGF-α: 0.49 - 2,000
  • Perforin: 97.66 - 400,000
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions• Recommended storage for kit components is 2 - 8°C.
• For long-term storage, freeze reconstituted standards and controls at -20°C. Avoid multiple freeze/thaw cycles.
• DO NOT FREEZE Antibody-Immobilized Beads, Detection Antibody, and Streptavidin-Phycoerythrin.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PRCYASF-40K 04065267238135

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