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527460 PhosphoDetect™ Anti-PKR (pThr⁴⁵¹) Rabbit pAb

This PhosphoDetect™ Anti-PKR (pThr⁴⁵¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of PKR (pThr⁴⁵¹).

PhosphoDetect™ Anti-PKR (pThr⁴⁵¹) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Double-stranded RNA-dependent Protein Kinase, Phospho-Specific (Thr⁴⁵¹)

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527460
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

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      Description
      OverviewRecognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
      Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
      Catalogue Number527460
      Brand Family Calbiochem®
      SynonymsAnti-Double-stranded RNA-dependent Protein Kinase, Phospho-Specific (Thr⁴⁵¹)
      Application Data
      Detection of human PKR, phosphorylated on Thr 451, by immunoblotting. Samples: Whole cell lysates from HeLa cells left unstimulated (lane 1) or stimulated with IFNγ and calyculin (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-PKR (pThr 451) Rabbit pAb (Cat. No. 527460) (1:000) pre-incubated without peptide (lanes 1-2), non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
      References
      ReferencesCuddihy, A.R., et al.1999. Oncogene 8, 2690.
      Srivastava, S.P., et al. 1998. J. Biol. Chem. 273, 2416.
      Clemens, M.J. 1997. Int. J. Biochem. Cell Biol. 29, 945.
      Product Information
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg+2 or Ca+2), 0.1% BSA, 50% glycerol, pH 7.3.
      Positive controlIFNγ-stimulated HeLa cells.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesImmunoblotting (1:1000)
      Immunohistochemistry (see comments)
      Application CommentsWhen used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr⁴⁵¹ phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      527460-10T 04055977270396

      Documentation

      PhosphoDetect™ Anti-PKR (pThr⁴⁵¹) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-PKR (pThr⁴⁵¹) Rabbit pAb Certificates of Analysis

      TitleLot Number
      527460

      References

      Reference overview
      Cuddihy, A.R., et al.1999. Oncogene 8, 2690.
      Srivastava, S.P., et al. 1998. J. Biol. Chem. 273, 2416.
      Clemens, M.J. 1997. Int. J. Biochem. Cell Biol. 29, 945.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-August-2007 RFH
      SynonymsAnti-Double-stranded RNA-dependent Protein Kinase, Phospho-Specific (Thr⁴⁵¹)
      ApplicationImmunoblotting (1:1000)
      Immunohistochemistry (see comments)
      Application Data
      Detection of human PKR, phosphorylated on Thr 451, by immunoblotting. Samples: Whole cell lysates from HeLa cells left unstimulated (lane 1) or stimulated with IFNγ and calyculin (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-PKR (pThr 451) Rabbit pAb (Cat. No. 527460) (1:000) pre-incubated without peptide (lanes 1-2), non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phophorylation site, followed by immunoaffinity chromatography. Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
      BackgroundPKR is an interferon-inducible serine/threonine kinase activated by double stranded RNA. The activation of PKR is accompanied by phosphorylation at multiple sites, including Thr446 and Thr451 in the PKR activation loop. PKR phosphorylates the α-subunit of the eukaryotic initiation factor-2 and inhibits protein synthesis. PKR also phosphorylates p53 and plays a role in the interferon response to viral infections.
      HostRabbit
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr⁴⁵¹ phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)
      IsotypeIgG
      Specieshuman, mouse
      Positive controlIFNγ-stimulated HeLa cells.
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg+2 or Ca+2), 0.1% BSA, 50% glycerol, pH 7.3.
      Preservative≤0.1% sodium azide
      CommentsWhen used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesCuddihy, A.R., et al.1999. Oncogene 8, 2690.
      Srivastava, S.P., et al. 1998. J. Biol. Chem. 273, 2416.
      Clemens, M.J. 1997. Int. J. Biochem. Cell Biol. 29, 945.