Millipore Sigma Vibrant Logo

MAB1501 Anti-Actin Antibody, clone C4

View Products on Sigmaaldrich.com
MAB1501
100 µL  
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
 
Solicitar precio
Disponibilidad a confirmar
Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

      Ofertas especiales

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AELISA, ICC, IF, IHC, IH(P), WBMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMAB1501
      Replaces04-1040
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Actin Antibody, clone C4
      Alternate Names
      • MAB1501X
      • MAB1501R
      Background InformationActins are ubiquitous eukaryotic proteins that serve as multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, transcriptional regulation, and vesicle trafficking. These functions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues.
      Beta- and gamma-actins, also known as cytoplasmic actins, are highly conserved in higher animals and are predominantly expressed in non-muscle cells where they control cell structure and motility. They are nearly identical proteins and differ only in four amino acids at the N-terminal region. The other four actin isoforms are typically found in specific adult muscle tissue types. alpha-cardiac and alpha-skeletal actins are expressed in striated cardiac and skeletal muscles, respectively. Alpha and gamma actins are primarily found in vascular smooth muscle and enteric smooth muscles, respectively. It has been shown that under calcium-bound conditions, beta-actin exhibits more dynamic behavior than gamma-actin with higher rates of polymerization and depolymerization. Also, beta- and gamma-actins can readily copolymerize, and the resulting filaments exhibit polymerization and depolymerization rates that vary depending on the ratio of beta- to gamma-actin (Lessard, JL., et al (1988). Cell Motility Cytoskeleton 10(3); 349-362).
      References
      Product Information
      FormatAscites
      HS Code3002 15 90
      Control
      • HeLa whole cell lysate.
      PresentationMouse monoclonal Ascites fluid, with 0.01% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationReliably and specifically detect actin using this Anti-Actin Antibody, clone C4. This highly published monoclonal antibody is validated for use in ELISA, IC, IF, IH, IH(P) & WB. This mAb is also available as a fluorescent conjugate.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesIndirect immunofluorescence at 1:100:
      Tissue culture cells -- fix with formaldehyde, treat with methanol or acetone.
      Glycerinated myofibrils -- fix fibers with formaldehyde, treat with cold methanol. Stains I-bands intensely and stress fibers in human fibroblasts.
      Cryostat sections (6 µm) -- quick frozen in isopentane, slides treated with gelatin and formaldehyde.

      Immunoblots:
      1:100-1:1,000 (Otey, 1987):On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43 kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha-actin found in skeletal, cardiac, and arterial muscle.

      Iodination (Lessard, 1979).

      Solid phase binding assay ELISA:
      1:800-1:1,000 dilution from a previous lot was shown to be strongly reactive with cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.

      ELISA:
      strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified chicken gizzard actin (Lessard, 1988).
      EpitopeEpitope is conserved in all known actins.
      CloneC4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMAB1501 is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts. Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).
      IsotypeIgG1κ
      Species Reactivity
      • All
      Species Reactivity NoteTo date, all animal species and cell types with an actin form react by indirect immunofluorescence or immunoblot, including plant actin.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
      Gene Symbol
      • ACTA1
      • MPFD
      • ASMA
      • NEM1
      • CFTDM
      • ACTA
      • CFTD
      • NEM2
      • Alpha-actin-1
      • CFTD1
      • NEM3
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P68133 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
      SIZE: 377 amino acids; 42051 Da
      SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID.
      SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
      DISEASE: SwissProt: P68133 # Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. Nemaline myopathy (NEM) is a form of congenital myopathy characterized by abnormal thread- or rod-like structures in muscle fibers on histologic examination. The clinical phenotype is highly variable, with differing age at onset and severity. & Defects in ACTA1 are a cause of congenital myopathy with excess of thin myofilaments (CM) [MIM:102610]. & Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
      SIMILARITY: SwissProt: P68133 ## Belongs to the actin family.
      MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
      Molecular Weight43 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on A431 lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected ACTIN on 10 ug of A431 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at -20°C in undiluted aliquots for up to 12 months from date of receipt. Do not store in a diluted format. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MAB1501 08436037122729

      Documentation

      Anti-Actin Antibody, clone C4 Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-Actin Antibody, clone C4 Certificados de análisis

      CargoNúmero de lote
      Anti-Actin, Clone C4 - 3261302 3261302
      Anti-Actin, Clone C4 - 3282532 3282532
      Anti-Actin, Clone C4 - 3423208 3423208
      Anti-Actin, Clone C4 - 3590048 3590048
      Anti-Actin, Clone C4 - 3698575 3698575
      Anti-Actin, Clone C4 - 3748816 3748816
      Anti-Actin, Clone C4 - 3800739 3800739
      Anti-Actin, Clone C4 - 3845682 3845682
      Anti-Actin, Clone C4 - 3872288 3872288
      Anti-Actin, Clone C4 - 3920772 3920772

      Referencias bibliográficas

      Visión general referenciasAplicación EspeciePub Med ID
      Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats.
      Pineda-Farias, JB; Barragán-Iglesias, P; Loeza-Alcocer, E; Torres-López, JE; Rocha-González, HI; Pérez-Severiano, F; Delgado-Lezama, R; Granados-Soto, V
      Molecular pain  11  41  2015

      Mostrar resumen
      26130088 26130088
      Novel Mechanisms of Spinal Cord Plasticity in a Mouse Model of Motoneuron Disease.
      Gulino, R; Parenti, R; Gulisano, M
      BioMed research international  2015  654637  2015

      Mostrar resumen
      26064939 26064939
      Bardoxolone Methyl Prevents Fat Deposition and Inflammation in Brown Adipose Tissue and Enhances Sympathetic Activity in Mice Fed a High-Fat Diet.
      Dinh, CH; Szabo, A; Yu, Y; Camer, D; Zhang, Q; Wang, H; Huang, XF
      Nutrients  7  4705-23  2015

      Mostrar resumen
      26066016 26066016
      Spinoculation Enhances HBV Infection in NTCP-Reconstituted Hepatocytes.
      Yan, R; Zhang, Y; Cai, D; Liu, Y; Cuconati, A; Guo, H
      PloS one  10  e0129889  2015

      Mostrar resumen
      26070202 26070202
      Involvement of cAMP-guanine nucleotide exchange factor II in hippocampal long-term depression and behavioral flexibility.
      Lee, K; Kobayashi, Y; Seo, H; Kwak, JH; Masuda, A; Lim, CS; Lee, HR; Kang, SJ; Park, P; Sim, SE; Kogo, N; Kawasaki, H; Kaang, BK; Itohara, S
      Molecular brain  8  38  2015

      Mostrar resumen
      26104314 26104314
      Regulation of chromatin accessibility and Zic binding at enhancers in the developing cerebellum.
      Frank, CL; Liu, F; Wijayatunge, R; Song, L; Biegler, MT; Yang, MG; Vockley, CM; Safi, A; Gersbach, CA; Crawford, GE; West, AE
      Nature neuroscience  18  647-56  2015

      Mostrar resumen
      25849986 25849986
      α-Actinin-4 enhances colorectal cancer cell invasion by suppressing focal adhesion maturation.
      Fukumoto, M; Kurisu, S; Yamada, T; Takenawa, T
      PloS one  10  e0120616  2015

      Mostrar resumen
      25860875 25860875
      Causal Modeling of Cancer-Stromal Communication Identifies PAPPA as a Novel Stroma-Secreted Factor Activating NFκB Signaling in Hepatocellular Carcinoma.
      Engelmann, JC; Amann, T; Ott-Rötzer, B; Nützel, M; Reinders, Y; Reinders, J; Thasler, WE; Kristl, T; Teufel, A; Huber, CG; Oefner, PJ; Spang, R; Hellerbrand, C
      PLoS computational biology  11  e1004293  2015

      Mostrar resumen
      26020769 26020769
      A novel fragile X syndrome mutation reveals a conserved role for the carboxy-terminus in FMRP localization and function.
      Okray, Z; de Esch, CE; Van Esch, H; Devriendt, K; Claeys, A; Yan, J; Verbeeck, J; Froyen, G; Willemsen, R; de Vrij, FM; Hassan, BA
      EMBO molecular medicine  7  423-37  2015

      Mostrar resumen
      25693964 25693964
      Exclusion of the unfolded protein response in light-induced retinal degeneration in the canine T4R RHO model of autosomal dominant retinitis pigmentosa.
      Marsili, S; Genini, S; Sudharsan, R; Gingrich, J; Aguirre, GD; Beltran, WA
      PloS one  10  e0115723  2015

      Mostrar resumen
      25695253 25695253

      Folleto

      Cargo
      AXIS: Axon Investigation System
      SNAP i.d. 2.0 System Brochure
      Western Blotting Tools

      Licencias necesarias e Información técnica

      Cargo
      Protein Blotting Handbook: 6th Edition

      Productos y aplicaciones relacionados

      Related Products

      Número de referencia Descripción  
      MAB1501R Anti-Actin Antibody,clone C4 Precios y disponibilidad

      Alternative Format

      Número de referencia Descripción  
      MAB1501R Anti-Actin Antibody,clone C4 Precios y disponibilidad

      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies