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382158

Anti-Histone H3, Acetylated (1-20) Rabbit pAb

liquid, Calbiochem®

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A propos de cet article

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
Tetrahymena sp., human
Application:
—
Citations:
6
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biological source

rabbit

Quality Segment

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

Tetrahymena sp., human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (10-500 ng/mL, chemiluminescence)
Immunocytochemistry (5 µg/mL)
Immunoprecipitation )

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

acetylation (Lys9,Lys14)

Gene Information

human ... H3C1(8350)

General description

Anti-Histone H3, Acetylated (1-20), rabbit polyclonal, recognizes the ~17 kDa acetylated histone H3 protein. It is validated for use in Western blotting, immunocytochemistry & immunoprecipitation.
Protein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa acetylated histone H3 protein.
Recognizes the ~17 kDa acetylated histone H3 protein.

Immunogen

Human
a diacetylated synthetic peptide [ARTKQTARAcKSTGGAcKAPRKQL(C)] corresponding to amino acids 1-20 of human histone H3, conjugated to KLH

Application

Immunoblotting (10-500 ng/ml, chemiluminescence)

Immunocytochemistry (5 µg/ml)

Immunoprecipitation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
HeLa cells

Other Notes

Braunstein, M., et al. 1996. Mol. Cell. Biol.16, 4349.
Braunstein, M., et al. 1993. Genes Devel.7, 592.
Perry, C.A., et al. 1993. Biochemistry32, 13605.
Lin, R., et al. 1989. J. Cell. Biol.108, 1577.
For immunocytochemistry, HeLa cells were fixed with 95% ethanol/5% acetic acid and treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h. 10-500 ng/ml of antibody detected acetylated histone H3 in acid-extracted proteins from HeLa cells. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H3. Higher concentrations of antibody also detected unacetylated histone H3. Preliminary evidence from an independent library suggests that phosphorylation of Ser10 on histone H3 may disrupt epitope recognition by this antibody. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Classe de stockage

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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