Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN2440 Anti-ABCA4 Antibody, clone 5B4

Anti-ABCA4, clone 5B4, Cat. No. MABN2440, is a highly specific mouse monoclonal antibody that targets Retinal-specific ATP-binding cassette transporter and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Anti-ABCA4 Antibody, clone 5B4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: ABCA4, ABCR UniProt Number: P78363
MABN2440
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, HICC, WB, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2440
DescriptionAnti-ABCA4 Antibody, clone 5B4
Alternate Names
  • Retinal-specific ATP-binding cassette transporter
  • ATP-binding cassette sub-family A member 4
  • RIM ABC transporter
  • RIM protein
  • RmP
  • Stargardt disease protein
Background InformationRetinal-specific ATP-binding cassette transporter (UniProt: P78363; also known as ATP-binding cassette sub-family A member 4, ABCA4, RIM ABC transporter, RIM protein, RmP, Stargardt disease protein) is encoded by the ABCA4 (also known as ABCR) gene (Gene ID: 24) in human. ABCA4 is a multi-pass membrane protein that is exclusively expressed in retina and is found in the rims of rod photoreceptor cells. It contains two ABC transporter domains (aa 929-1160 and 1938-2170). In the visual cycle, it act as a retinal extruder or retinal-phosphatidylethanolamine flippase to facilitate the removal of all-trans-retinal from disc membranes following the photobleaching of rhodopsin. Mutations in ABCA4 gene are reported to cause Stargardt disease, which is characterized by decreased central vision, atrophy of the macula and underlying retinal pigment epithelium, and frequent presence of prominent flecks in the posterior pole of the retina. It has also been linked to age-related macular degeneration and retinitis pigmentosa.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-ABCA4, clone 5B4, Cat. No. MABN2440, is a highly specific mouse monoclonal antibody that targets Retinal-specific ATP-binding cassette transporter and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunocytochemistry Analysis: A representative lot detected ABCA4 in Immunocytochemistry applications (Ahn, J., et. al. (2003). J Biol Chem. 278(41):39600-8).

Western Blotting Analysis: A representative lot detected ABCA4 in Western Blotting applications (Ahn, J., et. al. (2003). J Biol Chem. 278(41):39600-8; Bungert, S., et. al. (2001). J Biol Chem. 276(26):23539-46).

Immunoprecipitation Analysis: A representative lot detected ABCA4 in Immunoprecipitation applications (Ahn, J., et. al. (2003). J Biol Chem. 278(41):39600-8; Bungert, S., et. al. (2001). J Biol Chem. 276(26):23539-46).
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 249 amino acids from the N-terminal half of human Retinal-specific ATP-binding cassette transporter.
Clone5B4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5B4 detects Retinal-specific ATP-binding cassette transporter protein in human and bovine. It targets an epitope within 249 amino acids from the N-terminal half.
IsotypeIgG2bκ
Species Reactivity
  • Bovine
  • Human
Species Reactivity NoteHuman, Bovine. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ABCA4
  • ABCR
Purification MethodProtein G purified
UniProt Number
Molecular Weight250 kDa observed; 255.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human retina tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ABCA4 in 10 µg of human retina tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2440 04054839396724

Documentation

Anti-ABCA4 Antibody, clone 5B4 Certificates of Analysis

TitleLot Number
Anti-ABCA4, clone 5B4 - 3217761 3217761
Anti-ABCA4, clone 5B4 - 3384381 3384381
Anti-ABCA4, clone 5B4 - 3503857 3503857
Anti-ABCA4, clone 5B4 - 3593693 3593693
Anti-ABCA4, clone 5B4 - 3777986 3777986
Anti-ABCA4, clone 5B4 - 3863363 3863363
Anti-ABCA4, clone 5B4 - 4017399 4017399
Anti-ABCA4, clone 5B4 - 4027399 4027399
Anti-ABCA4, clone 5B4 - 4132489 4132489
Anti-ABCA4, clone 5B4 - 4238476 4238476
Show more