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MABC697 Anti-ACLY, clone 5F8D11 Antibody

This Anti-ACLY, clone 5F8D11 Antibody is validated for use in western blotting, IHC, immunofluorescence, flow cytometry & ELISA for the detection of ACLY.

Anti-ACLY, clone 5F8D11 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: ACLY UniProt Number: P53396
MABC697
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, MkWB, IHC, IF, FC, ELISAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC697
DescriptionAnti-ACLY, clone 5F8D11 Antibody
Alternate Names
  • ATP-citrate synthase
  • ATP-citrate (pro-S-)-lyase
  • ACL
  • Citrate cleavage enzyme
Background InformationATP citrate synthase, also known as ATP-citrate (pro-S-)-lyase (ACL) or Citrate cleavage enzyme, and encoded by the gene name ACLY is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal in buffer containing PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.
Quality LevelMQ100
Applications
ApplicationThis Anti-ACLY, clone 5F8D11 Antibody is validated for use in western blotting, IHC, immunofluorescence, flow cytometry & ELISA for the detection of ACLY.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunofluorescence
  • Flow Cytometry
  • ELISA
Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ACLY in esophageal cancer tissue.

Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected ACLY in HeLa cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected ACLY in HeLa cells.

ELISA: A 1:10,000 dilution from a representative lot was used on control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Biological Information
ImmunogenPurified recombinant fragment of human ACLY expressed in E. Coli
Clone5F8D11
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
  • Monkey
Antibody TypeMonoclonal Antibody
Gene Symbol
  • ACLY
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~125 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa, NIH/3T3, C6, COS7, and Raji cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ACLY in HeLa, NIH/3T3, C6, COS7, and Raji cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC697 04053252955259