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ABC1696 Anti-APH-1A Antibody

Anti-APH-1A Antibody is an antibody against APH-1A for use in Western Blotting.

Anti-APH-1A Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: APH1A, PSF, CGI-78, UNQ579/PRO1141 UniProt Number: Q96BI3
ABC1696
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC1696
DescriptionAnti-APH-1A Antibody
Alternate Names
  • Gamma-secretase subunit APH-1A
  • Anterior pharynx defective 1 homolog A
  • APH-1a
  • Aph-1alpha
  • Presenilin-stabilization factor
Background InformationGamma-secretase subunit APH-1A (UniProt Q96BI3; also known as Anterior pharynx defective 1 homolog A, APH-1a, Aph-1alpha, Presenilin-stabilization factor) is encoded by the APH1A (also known as PSF, CGI-78) gene (ORF UNQ579/PRO1141; Gene ID 51107) in human. Gamma-secretase catalyzes the final cleavage of beta-amyloid precursor protein to generate amyloid-beta peptide, the principal component of amyloid plaques found in Alzheimer's diseased (AD) brains. Gamma-secretase is a complex composed of four integral membrane proteins, presenilin (PS), nicastrin (NCT), PEN-2, and APH-1. The PS component could be either a PS1 or PS2 and the APH component could be either an APH-1a or APH-1b, alternative splicings of these four proteins contribute to further heterogeneity among gamma-secretase complexes. The gamma-secretase complex is assembled in the ER, where NCT and APH-1 form an initial scaffold for the PS holoprotein, which becomes stabilized upon interaction with the NCT/APH-1. Subsequent addition of PEN-2 to this trimeric assembly intermediate triggers the endoproteolytic cleavage of the PS holoprotein. The fourth transmembrane domain (TMD4) of APH-1, the PEN-2 C-terminal end, the NCT TMD domain, and the PS1 C-terminal end are shown to be essential for functional gamma-secretase complex assembly. APH-1a is a seven-transmembrane (a.a. 3-23, 32-52, 69-89, 119-139, 159-179, 187-207, 214-234) protein with a lumenal N-terminus and a cytoplasmic C-terminal tail (a.a. 235-265).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-APH-1A Antibody is an antibody against APH-1A for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a sequence from the C-terminal cytoplasmic tail of human APH-1A.
EpitopeC-terminal cytoplasmic tail.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityExpected to react with all three spliced isoforms of human APH-1A reported by UniProt (Q96BI3).
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat. Predicted to react with Mouse, Monkey based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • APH1A
  • PSF
  • CGI-78
  • UNQ579/PRO1141
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~32 kDa observed. 29.00 kDa (isoform 1; isoform L; isoform Alph-alpha1), 26.84 kDa (isoform 2; isoform S; isoform Alph-alpha2), 20.98 kDa (isoform 3) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected APH-1A in 10 µg of human kidney tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC1696 04055977332315