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CP01 Anti-Actin (Ab-1) Mouse mAb (JLA20)

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.

Anti-Actin (Ab-1) Mouse mAb (JLA20) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CP01
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
CP01-1EACN Plastic ampoule 1 ea
Description
OverviewRecognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.
  • Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1
  • Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN
  • Entrez Gene Name: actin, alpha 1, skeletal muscle
  • Hu Entrez ID: 58, 71 (Related Antibodies: CP64)
  • Mu Entrez ID: 11459 ;-; 11465
  • Rat Entrez ID: 29437 ;-; 287876
    		•
    Catalogue NumberCP01
    Brand Family Calbiochem®
    References
    ReferencesLin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
    Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
    Lin, J.J. 1981. Proc. Natl. Acad. Sci. 78, 2335.
    Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
    Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
    Product Information
    FormLiquid
    Formulation100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.
    Positive controlAll animal cell lines
    Preservative≤0.1% sodium azide (actin antibody only, CP01-100UL)
    Quality LevelMQ100
    Applications
    Application ReferencesImmunoblotting Thomas, W.D., et al. 2000. J. Immunol. 165, 5612.
    Key Applications Immunoblotting (Western Blotting)
    Immunofluorescence
    Application NotesImmunoblotting (1:5000-1:20000)
    Immunofluorescence (1:10000, see application references)

    Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
    Application CommentsActin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
    Biological Information
    Immunogenchicken cytoskeletal proteins
    ImmunogenChicken
    CloneJLA20
    HostMouse
    IsotypeIgM
    Species Reactivity
    • A Broad Range Of Species
    Antibody TypeMonoclonal Antibody
    Physicochemical Information
    Dimensions
    Materials Information
    Toxicological Information
    Safety Information according to GHS
    Safety Information
    Product Usage Statements
    Storage and Shipping Information
    Ship Code Dry Ice Only
    Toxicity Multiple Toxicity Values, refer to MSDS
    Storage -20°C
    Do not freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot ascites and freeze (-20°C).
    Packaging Information
    Transport Information
    Supplemental Information
    Specifications
    Global Trade Item Number
    Catalogue Number GTIN
    CP01-1EACN 07790788053697

    Documentation

    Anti-Actin (Ab-1) Mouse mAb (JLA20) SDS

    Title

    Safety Data Sheet (SDS) 

    Anti-Actin (Ab-1) Mouse mAb (JLA20) Certificates of Analysis

    TitleLot Number
    CP01

    References

    Reference overview
    Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
    Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
    Lin, J.J. 1981. Proc. Natl. Acad. Sci. 78, 2335.
    Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
    Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.

    Brochure

    Title
    Antibody Sourcebookﾙ Sixth Edition

    Citations

    Title
  • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision29-October-2008 JSW
    ApplicationImmunoblotting (1:5000-1:20000)
    Immunofluorescence (1:10000, see application references)

    Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
    DescriptionMouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.
    BackgroundCytoskeletal proteins are found in highly organized arrays within the cytoplasm of higher eukaryotic cells. These proteins are intimately involved in functions such as cell and intracellular organelle movement, maintenance of cell shape and endocytosis. The cytoskeleton of most eukaryotic cells is comprised of at least three fibrous systems each with accessory proteins: the microfilaments, composed of actin; the microfibrils, composed of tubulin; and the intermediate filaments composed of vimentin. Monoclonal antibodies have been used to study the components of the cytoskeleton and their intracellular location, changes in the expression of these elements during differentiation, and the function of the cytoskeleton in living cells by microinjection of the antibodies.
    HostMouse
    Immunogen speciesChicken
    Immunogenchicken cytoskeletal proteins
    CloneJLA20
    IsotypeIgM
    Speciesa broad range of species
    Positive controlAll animal cell lines
    FormLiquid
    Formulation100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.
    Preservative≤0.1% sodium azide (actin antibody only, CP01-100UL)
    CommentsActin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
    Storage -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot ascites and freeze (-20°C).
    Toxicity Multiple Toxicity Values, refer to MSDS
    ReferencesLin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
    Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
    Lin, J.J. 1981. Proc. Natl. Acad. Sci. 78, 2335.
    Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
    Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
    Citation
  • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
    		•
    Application referencesImmunoblotting Thomas, W.D., et al. 2000. J. Immunol. 165, 5612.