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MAB1501-AF555 Anti-Actin Antibody, clone C4, Alexa Fluor® 555 Conjugate

This Anti-Actin Antibody, clone C4, Alexa Fluor® 555 Conjugate is validated for use in Immunocytochemistry for the detection of Actin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: ACTA1, ACTA UniProt Number: P68139
MAB1501-AF555
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCMAlexaFluor®647Monoclonal Antibody
Description
Catalogue NumberMAB1501-AF555
DescriptionAnti-Actin Antibody, clone C4, Alexa Fluor® 555 Conjugate
Alternate Names
  • Actin, alpha skeletal muscle
  • Alpha-actin-1
  • Actin
Background InformationActins are ubiquitous eukaryotic proteins that serve as multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, transcriptional regulation, and vesicle trafficking. These functions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues.
Beta- and gamma-actins, also known as cytoplasmic actins, are highly conserved in higher animals and are predominantly expressed in non-muscle cells where they control cell structure and motility. They are nearly identical proteins and differ only in four amino acids at the N-terminal region. The other four actin isoforms are typically found in specific adult muscle tissue types. alpha-cardiac and alpha-skeletal actins are expressed in striated cardiac and skeletal muscles, respectively. Alpha and gamma actins are primarily found in vascular smooth muscle and enteric smooth muscles, respectively. It has been shown that under calcium-bound conditions, beta-actin exhibits more dynamic behavior than gamma-actin with higher rates of polymerization and depolymerization. Also, beta- and gamma-actins can readily copolymerize, and the resulting filaments exhibit polymerization and depolymerization rates that vary depending on the ratio of beta- to gamma-actin (Lessard, JL., et al (1988). Cell Motility Cytoskeleton 10(3); 349-362).
References
Product Information
FormatAlexaFluor®647
PresentationPurified mouse antibody conjugate in buffer containing PBS, 15 mg/mL BSA, and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Actin Antibody, clone C4, Alexa Fluor® 555 Conjugate is validated for use in Immunocytochemistry for the detection of Actin.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Actin in A431, and NIH/3T3 cells.
The unconjugated version of this antibody (Cat. No. MAB1501) has been demonstrated to perform in WB, ELISA, ICC, IF, IHC, IH(P).
Biological Information
ImmunogenPurified chicken gizzard actin (Lessard, 1988).
EpitopeEpitope is conserved in all known actins.
CloneC4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityMAB1501 is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts. Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Chicken based on 100% sequence homology. To date, all animal species and cell types with an actin form react by indirect immunofluorescence or immunoblot, including plant actin.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ACTA1
  • ACTA
Purification MethodProtein G Purified
UniProt Number
Molecular Weight40 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Actin in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB1501-AF555 04055977182033

Documentation

Anti-Actin Antibody, clone C4, Alexa Fluor® 555 Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-Actin Antibody, clone C4, Alexa Fluor® 555 Conjugate Certificates of Analysis

TitleLot Number
Anti-Actin, clone C4, Alexa Fluor® 555 Conjugate - 3231525 3231525
Anti-Actin, clone C4, Alexa Fluor® 555 Conjugate - 3872371 3872371
Anti-Actin, clone C4, Alexa Fluor® 555 Conjugate -2722594 2722594
Anti-Actin, clone C4, Alexa Fluor® 555 Conjugate -Q2586382 Q2586382
Anti-Actin, clone C4, Alexa Fluor®555 Conjugate 2977853

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.