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MABE1141-100UG Anti-CD3D (TCR δ) Antibody, clone 5A6.E9

MABE1141-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABE1141-100UG
DescriptionAnti-CD3D (TCR δ) Antibody, clone 5A6.E9
Alternate Names
  • T-cell surface glycoprotein CD3 delta chain
  • T-cell receptor T3 delta chain
  • CD3d
Background InformationT-cell surface glycoprotein CD3 delta chain (UniProt: P04234; also known as T-cell receptor T3 delta chain, CD3d) is encoded by the CD3D (also known as T3D) gene (Gene ID: 915) in human. CD3d is a single-pass type I membrane protein that is part of the TCR-CD3 complex present on T-lymphocyte cell surface. It plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs are phosphorylated by LCK and FYN resulting in the activation of downstream signaling pathways. CD3d is also reported to be involved in thymocyte differentiation and in the absence of a functional TCR-CD3 complex, thymocytes do not differentiate properly. CD3d also interacts with CD4 and CD8 to establish a functional link between the TCR and coreceptors CD4 and CD8, which is shown to be essential for activation and positive selection of CD4 or CD8 T-cells. CD3d is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 106-126), a transmembrane domain (aa 106-126), and a cytoplasmic domain (aa 127-171).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Applications
ApplicationAnti-CD3d (TCR d), clone 5A6.E9, Cat. No. MABE1141, is a mouse monoclonal antibody that detects T-cell surface glycoprotein CD3 delta chain and is tested for use in Flow Cytometry, Immunohistochemistry, and Inhibition analysis.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
  • Inhibition
Application NotesImmunohistochemistry Analysis: A representative lot detected CD3d in Immunohistochemistry applications (Takeshita, M., et. al. (2004). Am J Clin Pathol. 121(3):408-16).

Inhibition Analysis: A representative lot blocked CD3d receptor and induced apoptosis. (Dutta, I., et. al. (2017). Front Immunol. 8: Article 776).

Flow Cytometry Analysis: A representative lot detected CD3d in Flow Cytometry applications (Van Rhijn, I., et. al. (2003). Int Immunol. 15(3):373-82; Neves, P.C., et. al. (2010). Vaccine. 28(51):8183-8).
Biological Information
ImmunogenHuman leukemic (T-ALL) PEER cells.
Epitopeextracellular domain
Clone5A6.E9
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 5A6.E9 is a mouse monoclonal antibody that detects T-cell surface glycoprotein CD3 delta chain (CD3D). It targets an epitope within the extracellular domain.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD3D
  • T3D
Purification MethodProtein G purified
UniProt Number
Molecular Weight20.47 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in PEER cells.

Flow Cytometry Analysis: 2 µg of this antibody detected CD3d in one million PEER cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1141-100UG 04061841762065

Documentation

Anti-CD3D (TCR δ) Antibody, clone 5A6.E9 SDS

Title

Safety Data Sheet (SDS) 

Anti-CD3D (TCR δ) Antibody, clone 5A6.E9 Certificates of Analysis

TitleLot Number
Anti-CD3G (TCR γ/δ), clone 5A6.E9 - 4036283 4036283
Anti-CD3G (TCR γ/δ), clone 5A6.E9 - 4134982 4134982
Anti-CD3G (TCR γ/δ), clone 5A6.E9 - 4143313 4143313
Anti-CD3G (TCR γ/δ), clone 5A6.E9 - 4187680 4187680
Anti-CD3G (TCR γ/δ), clone 5A6.E9 - 4272804 4272804
Anti-CD3G (TCR γ/δ), clone 5A6.E9 - Q3430110 Q3430110

References

Reference overviewPub Med ID
Apoptosis Induced via Gamma Delta T Cell Antigen Receptor "Blocking" Antibodies: A Cautionary Tale.
Dutta, I; Postovit, LM; Siegers, GM
Front Immunol  8  776  2017

Show Abstract
28713391 28713391
CD8+ gamma-delta TCR+ and CD4+ T cells produce IFN-γ at 5-7 days after yellow fever vaccination in Indian rhesus macaques, before the induction of classical antigen-specific T cell responses.
Neves, PC; Rudersdorf, RA; Galler, R; Bonaldo, MC; de Santana, MG; Mudd, PA; Martins, MA; Rakasz, EG; Wilson, NA; Watkins, DI
Vaccine  28  8183-8  2010

Show Abstract
20939995 20939995
Clinicopathologic analysis of 22 cases of subcutaneous panniculitis-like CD56- or CD56+ lymphoma and review of 44 other reported cases.
Takeshita, M; Imayama, S; Oshiro, Y; Kurihara, K; Okamoto, S; Matsuki, Y; Nakashima, Y; Okamura, T; Shiratsuchi, M; Hayashi, T; Kikuchi, M
Am J Clin Pathol  121  408-16  2004

Show Abstract
15023046 15023046
Expansion of human gammadelta T cells after in vitro stimulation with Campylobacter jejuni.
Van Rhijn, I; Van den Berg, LH; Ang, CW; Admiraal, J; Logtenberg, T
Int Immunol  15  373-82  2003

Show Abstract
12618481 12618481