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ABS2088 Anti-CLIC4

Detect Chloride intracellular channel protein 4 using this rabbit polyclonal Anti-CLIC4, Cat. No. ABS2088, used in Western Blotting and Immunofluorescence, Western Blotting, Immunohistochemistry (Paraffin) and Immunoprecipitation.

Anti-CLIC4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: CLIC4 UniProt Number: Q9XSA7
ABS2088
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWB, IF, IH(P), IPRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABS2088
DescriptionAnti-CLIC4
Alternate Names
  • Intracellular chloride ion channel protein p64H1
Background InformationChloride intracellular channel protein 4 (UniProt: Q9XSA7; also known as Intracellular chloride ion channel protein p64H1, CLIC4) is encoded by the CLIC4 gene (Gene ID: 286823) in bovine. CLIC4, a member of the CLIC family of proteins. CLIC family members can change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion. CLIC4 is ubiquitously expressed and is present in both soluble and membrane-associated forms. It is present in the cytosol as a diffusible protein, but is reported in endoplasmic reticulum, trans-Golgi network, mitochondria, caveolae, dense-core secretory vesicles in the central nervous system, and in nuclei of differentiating keratinocytes. CLIC4 is rapidly recruited to the plasma membrane by lysophosphatidic acid (LPA) and serum, indicating its possible role in exocytic endocytic trafficking. CLIC4 also participates in such diverse processes as angiogenesis, differentiation, migration and wound healing. It is reported to stimulate both the internalization and serum/ LPA-induced recycling of beta1 integrin. In developing and developed kidneys, CLIC4 is enriched in the proximal tubule epithelial cells where it is considered to be essential for luminal delivery, microvillus morphogenesis, and endo-lysosomal biogenesis. CLIC4-null mice embryos exhibit impaired renal tubulogenesis and have smaller kidneys with fewer glomeruli and less dense peritubular capillary network. (Ref.: Edwards, JC et al. (2014). BMC Nephrology 15:54; Argenzio, A et al. (2014). J. Cell Sci.127 (24): 5189-5203).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.02% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Chloride intracellular channel protein 4 using this rabbit polyclonal Anti-CLIC4, Cat. No. ABS2088, used in Western Blotting and Immunofluorescence, Western Blotting, Immunohistochemistry (Paraffin) and Immunoprecipitation.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: A representative lot detected CLIC4 in Madin-Darby canine kidney (MDCK) cells (Chou, S.Y., et. al. (2016). Nat Commun. 7:10412).

Immunohistochemistry Analysis: A representative lot detected CLIC4 in Immunohistochemistry applications (Chou, S.Y., et. al. (2016). Nat Commun. 7:10412).

Western Blotting Analysis: A representative lot detected CLIC4 in immunoblots of renal lysates harvested from WT and CLIC4-KO postnatal 0 (PN0 mice) (Chou, S.Y., et. al. (2016). Nat Commun. 7:10412).

Immunofluorescence Analysis: A representative lot detected CLIC4 in postnatal 0 (PN0) mouse renal paraffin and frozen sections (Chou, S.Y., et. al. (2016). Nat Commun. 7:10412).
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 158 amino acids from the internal region of bovine Chloride intracellular channel protein 4 (CLIC4).
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects intracellular channel protein 4 in rat brain.
Species Reactivity
  • Rat
Species Reactivity NoteRat. Predicted to react with Bovine.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CLIC4
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~29 kDa observed; 28.73 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1:500 dilution of this antibody detected CLIC4 in 10 µg of rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS2088 04054839168734