Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MAB5432-C Anti-CREB Antibody, clone LB9, Ascites Free

Anti-CREB Antibody, clone LB9, Ascites Free is an antibody against CREB for use in Western Blotting, Immunohistochemistry (Paraffin).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Transcription Factors Gene Symbol: CREB1 UniProt Number: P16220
MAB5432-C
100 μg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB5432-C
DescriptionAnti-CREB Antibody, clone LB9, Ascites Free
Alternate Names
  • Cyclic AMP-responsive element-binding protein 1
  • cAMP-responsive element-binding protein 1
  • CREB-1
Background InformationCyclic AMP-responsive element-binding protein 1 (UniProt P16220; also known as cAMP-responsive element-binding protein 1, CREB-1) is encoded by the CREB1 gene (Gene ID 1385) in human. CREB is a transcription factor that binds the cAMP response elements (CRE) and regulates the transcription of the downstream genes. CREB activity is regulation via phosphorylation on by various kinases, including PKA and Ca2+/calmodulin-dependent protein kinases. Genes known to be regulated by CREB include c-fos, BDNF, tyrosine hydroxylase, neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone), as well as the circadian clock genes PER1 and PER2. CREB is critical for memory formation across a wide range of species.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CREB Antibody, clone LB9, Ascites Free is an antibody against CREB for use in Western Blotting, Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 1.0 µg/mL of this antibody from a representative lot detected CREB in 10 µg of NIH/3T3 and PC12 cell lysate.
Immunohistochemistry Analysis: An 1:50-250 dilution of this antibody from a representative lot detected CREB in human liver, colon, and tonsil tissue sections.
Western Blotting Analysis: Representative lots detected CREB in primary mouse astrocytes and mouse dorsal hippocampal tissue lysates (Orr, A.G., et al. (2015). Nat. Neurosci. 18(3):423-434; Yiu, A.P., et al. (2011). Neuropsychopharmacology. 36(11):2169-2186).
Western Blotting Analysis: Representative lots detected CREB in primary human fibroblast and SH-SY5Y human neuroblastoma cell lysates (Maj, M., et al. (2011). Mitochondrion. 11(2):264-272; Fukuda, Y., et al. (2010). Mol. Cell Neurosci. 45(3):226-233).
Immunohistochemistry Analysis: A representative lot immunostained total CREB in dorsal hippocampal tissue sections from paraformaldehyde-perfused mice (Yiu, A.P., et al. (2011). Neuropsychopharmacology. 36(11):2169-2186).
Biological Information
ImmunogenRecombinant CREB-A protein.
CloneLB9
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone LB9 reactivity towarad spliced isoform CREB-B and htCREB (isoform 3) has not been determined.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CREB1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~37-43 kDa observed. 36.69 kDa (isoform CREB-A) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected CREB in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB5432-C 04055977330205

Related Products & Applications

Related Products

Catalogue Number Description
04-767 Anti-CREB Antibody, clone NL904, rabbit monoclonal
05-667 Anti-phospho-CREB (Ser133) Antibody, clone 10E9
05-807 Anti-phospho-CREB (Ser133) Antibody, clone 634-2
06-519 Anti-phospho-CREB (Ser133) Antibody
06-519-AF488 Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor® 488 conjugate
06-519-AF647 Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor®647 conjugate
06-863 Anti-CREB Antibody
17-10131 ChIPAb+™ Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer Set
17-600 ChIPAb+ CREB - ChIP Validated Antibody and Primer Set, rabbit monoclonal
AB3006 Anti-CREB Antibody
Show more

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies