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MABN695 Anti-CRTC2 Antibody, clone 5B10

Anti-CRTC2 Antibody, clone 5B10 is a highly specific mouse monoclonal antibody, that targets CREB-regulated transcription coactivator 2 & has been tested in western blotting, ICC, IHC & Flow Cytometry.

Anti-CRTC2 Antibody, clone 5B10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: TORC2, TORC-2, CRTC2 UniProt Number: Q53ET0
MABN695
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, IHC, FCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABN695
DescriptionAnti-CRTC2 Antibody, clone 5B10
Alternate Names
  • TORC2
  • TORC-2
  • CRTC2
  • CREB-regulated transcription coactivator 2
  • Transducer of regulated cAMP response element-binding protein 2
  • Transducer of CREB protein 2
Background InformationCREB-regulated transcription coactivator 2 (CRTC2) is a transcriptional coactivator for CREB1 which is a cAMP (CRE) site responder. CRTC2 is also known as TORC-2 (transducer of regulated cAMP response element-binding protein 2) and CRTC2 regulates gluconeogenesis as well as steroidogenic processes, and is also a coactivator for mitochondrial biogenesis in muscle cells. Localization is cytoplasmic but relocates to the nucleus in response to cAMP and glucagon levels. CRTC2 is highly expressed in thymus, spleen, muscle, and lung; it is also expressed in various cancers as well as insulinomas. Low levels of expression are found in the brain, colon, heart and kidney, with the lowest levels being in the liver and pancreas. Activity is regulated via phosphorylation. CRTC2 is inactive when phosphorylated (meaning that is does not bind CREB and assist in gene transcription) and active when dephosphorylated. EMD-Millipore’s Anti-CRTC2 mouse monoclonal, clone 5B10, has been tested in western blots against human HEK292 and HeLa cell extracts, in immunohistochemistry in paraffin embedded human ovary and lung cancer tissue, and in immunofluorescent immunocytochemistry and flow cytometry on HeLa cells in culture.
References
Product Information
FormatAscites
Control
  • HeLa cell lysate
PresentationMouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CRTC2 Antibody, clone 5B10 is a highly specific mouse monoclonal antibody, that targets CREB-regulated transcription coactivator 2 & has been tested in western blotting, ICC, IHC & Flow Cytometry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
  • Flow Cytometry
Application NotesImmunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CRTC2 in HeLa cells.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CRTC2 in human lung cancer and ovarian tumor tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CRTC2 in HeLa cells.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified protein fragment of human CRTC2 expressed in E. coli.
Clone5B10
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TORC2
  • TORC-2
  • CRTC2
Purification MethodUnpurified
UniProt Number
Molecular Weight~76 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CRTC2 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN695 04053252922510

Documentation

Anti-CRTC2 Antibody, clone 5B10 SDS

Title

Safety Data Sheet (SDS) 

Anti-CRTC2 Antibody, clone 5B10 Certificates of Analysis

TitleLot Number
Anti-CRTC2, clone 5B10 - VP1404121 VP1404121
Anti-CRTC2, clone 5B10 - VP1911150 VP1911150
Anti-CRTC2, clone 5B10 - VP2207130 VP2207130
Anti-CRTC2, clone 5B10 -VP1501023 VP1501023
Anti-CRTC2, clone 5B10 -VP1601122 VP1601122
Anti-CRTC2, clone 5B10 -VP1606068 VP1606068
Anti-CRTC2, clone 5B10 -VP1611235 VP1611235
Anti-CRTC2, clone 5B10 Monoclonal Antibody VP1703249