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17-347B Anti-Citrulline (Modified) Detection Kit

The Anti-Citrulline (Modified) Detection Kit provides reagents for the modification and detection of citrulline-containing proteins by Western blot (immublot) analysis.

Anti-Citrulline (Modified) Detection Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology
17-347B
1 kit  Kit capacity: 10 western blots
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHost
AWBHuman Recombinant
Description
Catalogue Number17-347B
Replaces17-347
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Citrulline (Modified) Detection Kit
OverviewThe Anti-Citrulline (Modified) Detection Kit provides reagents for the modification and detection of citrulline-containing proteins by Western blot analysis. Modification of citrulline residues is created by a chemical reaction with 2,3-butanedione monoxime and antipyrine in a strong acid solution. Detection of the modified citrulline proteins uses a standard immunoblot protocol with an anti-Citrulline (modified) Human monoclonal antibody and a goat anti-Human IgG secondary antibody horseradish peroxidase (HRP) conjugate. This type of citrulline modification ensures the detection of citrulline residues in proteins regardless of neighboring amino acid sequences.
Materials Required but Not Delivered1. 98% H2SO4 (Sulfuric Acid)
2. 85% H3PO4 (Phosphoric Acid)
3. TBS-TWEEN® Tablets (EMD Millipore catalog #524753)
4. Luminata Crescendo Western HRP substrate (EMD Millipore catalog #WBLUR100)
5. Non-fat dry milk (EMD Millipore Catalog # 20-200)
Background InformationCitrullination or deimination is the post-translational modification of arginine residues to citrulline residues in proteins. This process is catalyzed by enzymes called peptidylarginine deiminases (PADs).
References
Product Information
Components
  • Anti-Modified Citrulline, clone C4 (Part number MABS487). One vial containing 100 µL of Protein A purified recombinant human monoclonal IgG1λ in PBS containing 0.05% sodium azide.
  • Goat Anti-Human IgG, HRP Conjugate: (Part number CS216591). One vial containing 50 µL of Goat anti-Human IgG conjugated to horseradish peroxidase (HRP) in PBS, pH 7.6, with 7.5 mg/mL BSA and diluted with Stabilzyme® and glycerol to 50%.
  • Reagent B: (Part number 20-253). One bottle containing 50 mL of 0.5% 2,3-butanedione monoxime, 0.25% antipyrine, 0.5 M acetic acid.
  • 0.5% FeCl3: (Part number 20-255). One bottle containing 4 mL of 0.5% FeCl3. Frozen solution.
HS Code3002 15 90
Quality LevelMQ300
Applications
ApplicationThe Anti-Citrulline (Modified) Detection Kit provides reagents for the modification and detection of citrulline-containing proteins by Western blot (immublot) analysis.
Key Applications
  • Western Blotting
Biological Information
HostHuman Recombinant
Species Reactivity
  • All
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore Anti-Modified Citrulline monoclonal antibody at 2-8°C. Store Goat Anti-Human IgG, HRP Conjugate, Reagent B, and 0.5% FeCl3 at -20°C for 4 months from date of receipt.
Packaging Information
Material Size1 kit
Material PackageKit capacity: 10 western blots
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
17-347B 04055977174137

Documentation

Anti-Citrulline (Modified) Detection Kit SDS

Title

Safety Data Sheet (SDS) 

Anti-Citrulline (Modified) Detection Kit Certificates of Analysis

TitleLot Number
Anti-Citrulline (Modified) Detection Kit - 3017763 3017763
Anti-Citrulline (Modified) Detection Kit - 3159042 3159042
Anti-Citrulline (Modified) Detection Kit - 3166769 3166769
Anti-Citrulline (Modified) Detection Kit - 3183436 3183436
Anti-Citrulline (Modified) Detection Kit - 3206073 3206073
Anti-Citrulline (Modified) Detection Kit - 3229599 3229599
Anti-Citrulline (Modified) Detection Kit - 3263094 3263094
Anti-Citrulline (Modified) Detection Kit - 3314359 3314359
Anti-Citrulline (Modified) Detection Kit - 3393749 3393749
Anti-Citrulline (Modified) Detection Kit - 3478349 3478349
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