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ABC138 Anti-DHODH Antibody

ABC138
100 µg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC138
DescriptionAnti-DHODH Antibody
Alternate Names
  • Dihydroorotate dehydrogenase (quinone), mitochondrial
  • DHOdehase
  • Dihydroorotate oxidase
Background InformationDihydroorotate oxidase (DHODH) is also called Dihydroorotate dehydrogenase (quinone), mitochondrial (DHOdehase). DHODH is a member the dihydroorotate dehydrogenase (Type 2) family and is involved in pyrimidine biosynthesis. DHODH catalyzes the conversion of dihydroorotate to orotate using quinone as an electron acceptor. DHODH defects can cause postaxial acrofacial dysostosis (POADS).
References
Product Information
FormatAffinity Purified
Control
  • T47D cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-DHODH Antibody is a rabbit polyclonal antibody, validated for use in western blotting & IHC (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:100 dilution from a representative lot detected DHODH in human testis and breast carcinoma sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the Topological Domain (Mitochondrial intermembrane) of human DHODH.
EpitopeTopological Domain ( Mitochondrial intermembrane)
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the Topological Domain (Mitochondrial intermembrane) of DHODH.
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human. Other Homologies: Rat (93% sequence homology) and Mouse (86% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • DHODH
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~43 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in T47D cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected DHODH in 10 µg of T47D cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC138 04053252915963