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MABE1877-100UG Anti-EBNA-1 Antibody, clone 1EB14

Anti-EBNA-1, clone 1EB14, Cat. No. MABE1877, is a mouse monoclonal antibody that detects Epstein-Barr nuclear antigen 1 and is tested in ELISA, Immunoprecipitation, and Western Blotting.

MABE1877-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABE1877-100UG
DescriptionAnti-EBNA-1 Antibody, clone 1EB14
Alternate Names
  • Epstein-Barr nuclear antigen 1
  • EBV nuclear antigen 1
Background InformationEpstein-Barr nuclear antigen 1 (UniProt: P03211; also known as EBNA-1, EBV nuclear antigen 1) is encoded by the EBNA1 (also known as BKRF1) gene (Gene ID: 3783709) in Epstein-Barr virus (EBV). EBV is a gamma-herpesvirus, a causative agent of infectious mononucleosis. EBNA1, a multi-functional protein, plays an important role in maintaining EBV in the host cell and has been implicated in host cell immortalization. It plays an essential role in replication and partitioning of viral genomic DNA during latent viral infection. During this phase, the circular double-stranded viral DNA undergoes replication once per cell cycle and is efficiently partitioned to the daughter cells. EBNA1 activates the initiation of viral DNA replication through binding to specific sites in the viral latent origin of replication, oriP. It is also reported to direct the segregation of viral episomes by mediating their attachment to host cell metaphase chromosomes and is known to activate the transcription of several viral latency genes. By counteracting the stabilization of host p53, it also reduces apoptosis and increases host cell survival. EBNA1 is also a risk factor in a variety of malignancies and is the only viral protein found in all EBV-related malignancies. Its C-terminal region contains a dimerization domain and a DNA binding domain (aa 459-607). Although this domain is essential for its replication function of EBNA1, it is not sufficient for this activity and the N-terminal half of EBNA1 is also required. (Ref.: Sun, X., et al. (2010). Proc. Natl. Acad. Sci. USA. 107(7); 3146-3151; Duellman, SJ., and Burgess, RR. (2009). PLoS ONE 4(2): e4614; Wu, H., et al. (2002). J. Virol. 76(5); 2480-2490).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-EBNA-1, clone 1EB14, Cat. No. MABE1877, is a mouse monoclonal antibody that detects Epstein-Barr nuclear antigen 1 and is tested in ELISA, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • ELISA
  • Immunoprecipitation
Application NotesTested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated EBNA-1 in Immunoprecipitation application. (Duellman, S.J., et al. (2009). PLoS One. 4(2):e4614).

ELISA Analysis: A representative lot detected EBNA-1 in ELISA application. (Duellman, S.J., et al. (2009). PLoS One. 4(2):e4614).

Western Blotting Analysis: A representative lot detected EBNA-1 in Western Blotting application. (Duellman, S.J., et al. (2009). PLoS One. 4(2):e4614; Duellman, S. J., et al. (2006). Protein Expr Purif. 47(2):434--40).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged recombinant Epstein-Barr nuclear antigen 1 expressed in E. coli.
EpitopeC-terminal half
Clone1EB14
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1EB14 is a mouse monoclonal antibody that detects Epstein-Barr nuclear antigen 1 (EBNA-1). It targets an epitope within 13 amino acids from the C-terminal half.
IsotypeIgG1κ
Species Reactivity
  • Virus
Species Reactivity NoteEpstein-Barr Virus.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • EBNA1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~60 kDa observed; 56.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected EBNA-1 in Raji cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1877-100UG 04065266876345

Documentation

Anti-EBNA-1 Antibody, clone 1EB14 SDS

Title

Safety Data Sheet (SDS) 

Anti-EBNA-1 Antibody, clone 1EB14 Certificates of Analysis

TitleLot Number
Anti-EBNA-1, clone 1EB14 - 4163798 4163798
Anti-EBNA-1, clone 1EB14 - 4164222 4164222
Anti-EBNA-1, clone 1EB14 - 4198943 4198943
Anti-EBNA-1, clone 1EB14 - 4230499 4230499
Anti-EBNA-1, clone 1EB14 - 4273728 4273728
Anti-EBNA-1, clone 1EB14 - 4288348 4288348
Anti-EBNA-1, clone 1EB14 - 4292213 4292213
Anti-EBNA-1, clone 1EB14 - Q3731094 Q3731094