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MABF851 Anti-ERAP1 Antibody, clone 16A7.1

Detect ERAP1 using this Anti-ERAP1 Antibody, clone 16A7.1 validated for use in Western Blotting.

Anti-ERAP1 Antibody, clone 16A7.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: ERAP1, APPILS, ARTS1, ERAAP, ERAAP1, KIAA0525, PILSAP UniProt Number: Q9NZ08
MABF851
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF851
DescriptionAnti-ERAP1 Antibody, clone 16A7.1
Alternate Names
  • Endoplasmic reticulum aminopeptidase 1
  • A-LAP
  • Adipocyte-derived leucine aminopeptidase
  • Aminopeptidase PILS
  • ARTS-1
  • Endoplasmic reticulum aminopeptidase associated with antigen processing
  • PILS-AP
  • Puromycin-insensitive leucyl-specific aminopeptidase
  • Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator
Background InformationEndoplasmic reticulum aminopeptidase 1 (UniProt Q9NZ08; also known as A-LAP, Adipocyte-derived leucine aminopeptidase, Aminopeptidase PILS, ARTS-1, Endoplasmic reticulum aminopeptidase associated with antigen processing, PILS-AP, Puromycin-insensitive leucyl-specific aminopeptidase, Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator) is encoded by the ERAP1 (also known as ALAP, APPILS, ARTS1, ERAAP, ERAAP1, KIAA0525, PILSAP) gene (ORF UNQ584/PRO1154; Gene ID 51752) in human. Endoplasmic reticulum aminopeptidase 1 (ERAP1) is a single transmembrane protein belonging to the M1 family of zinc metallopeptidases. ERAP1 consists of an N-terminal transmembrane segment (a.a. 2-21) and a large lumenal domain (a.a. 22-941), with only the N-terminus methionine exposed cytoplasmically. ERAP1 processes peptides for major histocompatibility complex (MHC) class I presentation and promotes ectodomain shedding of several cytokine receptors, including TNF-R1, IL-6R alpha chain, and IL-1 type II decoy receptor (IL-1RII). Ankylosing spondylitis (AS) is a common, highly heritable form of inflammatory arthritis. Several ERAP1 single nucleotide polymorphic (SNP) variants are reported to influence the risk of AS in individuals carrying class I MHC allele HLA-B*27 and HLA-B*40:01.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect ERAP1 using this Anti-ERAP1 Antibody, clone 16A7.1 validated for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected ERAP1 in 10 µg of K562 cell lysate.
Biological Information
ImmunogenGST-tagged recombinant human ERAP1 internal fragment.
EpitopeInternal.
Clone16A7.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with both spliced isoforms of human ERAP1 (UniProt Q9NZ08-1 & Q9NZ08-2)
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ERAP1
  • APPILS
  • ARTS1
  • ERAAP
  • ERAAP1
  • KIAA0525
  • PILSAP
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~110 kDa observed. 107.2 kDa (isoform 1) and 107.8 kDa (Isoform 2) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected ERAP1 in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF851 04055977312898