Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABE386 Anti-Flightless-1 homolog Antibody

Detect Fli1 using this rabbit polyclonal antibody, Anti-Flightless-1 homolog Antibody validated for use in western blotting & IP.

Anti-Flightless-1 homolog Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: FLII, FLIL UniProt Number: Q13045
ABE386
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IPRbSerumPolyclonal Antibody
Description
Catalogue NumberABE386
ReplacesMABF195; MABE530
DescriptionAnti-Flightless-1 homolog Antibody
Alternate Names
  • Protein flightless-1 homolog
  • Fli1
  • Anti-Flightless-1 homolog Antibody
Background InformationMammalian homolog of drosophila flightless-1 is encoded by the FLII gene in human (also known as FLI, Fli1, or FLIL). The human flightless-1 homolog (Flii) contains sixteen 23-amino acid leucine-rich repeats (LRR) in its N-terminal half and a gelsolin-like domain (GLD) in its C-terminal half, composed of six segmental repeats typical of many gelsolin family members. Flii is reported to interact with actin and "FLI LRR associated protein" (FLAP) via its GLD and LRR domain, respectively (PMID 9525888). Flii is also shown to interact with the glucose-responsive transcription factor carbohydrate responsive element binding protein (ChREBP) via both the LRR and GLD domains and functions as a negative regulator of ChREBP in cancer cells (PMID 24055811). Increased Flii level is observed in the blistered skin of patients with epidermolysis bullosa acquisita (EBA), and Flii overexpression in transgenic mice results in delayed epidermal barrier formation in embryos and decreased expression of tight junction (TJ) proteins, as well as impaired barrier function among adult transgenic mice (PMID 24375017).
References
Product Information
FormatSerum
PresentationRabbit polyclonal in buffer containing serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Fli1 using this rabbit polyclonal antibody, Anti-Flightless-1 homolog Antibody validated for use in western blotting & IP.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: A representative lot of this antibody detected Flightless-1 homolog in IP (Prof. D. Pallas, Emory University).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Flightless-1 homolog.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • FLII
  • FLIL
Purification MethodUnpurified
UniProt Number
Molecular Weight~144 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Flightless-1 homolog in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE386 04053252933868