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ABN51 Anti-Fox-3 Antibody

Anti-Fox-3, Cat. No. ABN51, is a highly specific rabbit polyclonal antibody that targets Fox 3/NeuN and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-Fox-3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: Rbfox3 UniProt Number: Q8BIF2
ABN51
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RWB, IH(P), ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN51
DescriptionAnti-Fox-3 Antibody
Alternate Names
  • RNA binding protein fox-1 homolog 3
  • Fox-1 homolog C
  • Hexaribonucleotide-binding protein 3
  • Neuronal nuclei antigen
  • NeuN antigen
Background InformationRNA binding protein fox-1 homolog 3 (UniProt: Q8BIF2; also known as Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Fox-3, Neuronal nuclei antigen, NeuN antigen) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID: 52897) in murine species. Fox-3 acts as a pre-mRNA alternative splicing regulator. It regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD). It is widely expressed in brain, including in cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, and in the spinal cord (at protein level). However, Purkinje cells lack Fox-3 expression. It is also found in the retina in the ganglion cells and some cells in the inner nuclear layer, but is absent from the photoreceptor cells and most cells in the inner nuclear layer. In developing mouse it is expressed in the neural tube as early as day E9.5. Fox-3 expression is shown to be up-regulated by retinoic acid in p19 cells during neural differentiation. Knockout mice display significantly reduced brain weight, impaired neurofilament expression and decreased white matter volume, but normal total body mass. Five isoforms of Fox-3 have been described that are generated by alternative splicing.
References
Product Information
FormatAffinity Purified
Control
  • Mouse e16 brain tissue lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Fox-3, Cat. No. ABN51, is a highly specific rabbit polyclonal antibody that targets Fox 3/NeuN and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected Fox-3 in mouse brain nuclear extract.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Fox-3 in mouse cerebral cortex, mouse hippocampus, and human cerebellum tissues.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Fox-3 in E18 rat cortex cells.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the N-terminus of mouse Fox-3.
EpitopeN-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes Fox-3 at the N-terminus.
Species Reactivity
  • Mouse
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Rbfox3
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: RNA-binding protein that regulates alternative splicing events (By similarity).

SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity).

SEQUENCE SIMILARITIES: Contains 1 RRM (RNA recognition motif) domain.
Molecular Weight~46 kDa observed. An uncharacterized band appears at ~68 kDa in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in mouse e16 brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Fox-3 on 10 µg of mouse e16 brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN51 04053252292415