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CB1001 Anti-GAPDH Mouse mAb (6C5)

Anti-GAPDH, mouse monoclonal, clone 6C5, recognizes ~146 kDa GAPDH protein under non-reducing conditions & 36 kDa monomers under reducing conditions. It is validated for use in ELISA, WB, and IHC.

Anti-GAPDH Mouse mAb (6C5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Glyceraldehyde-3-Phosphate Dehydrogenase

CB1001
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Ca, Ch, F, Fg, H, M, Porcine, Rb, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
CB1001-500UGCN Plastic ampoule 500 μg
Description
OverviewRecognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.
Catalogue NumberCB1001
Brand Family Calbiochem®
SynonymsAnti-Glyceraldehyde-3-Phosphate Dehydrogenase
Application Data
Detection of rat GAPDH by immunocytochemistry. Sample: A-10 cell line (rat aortic smooth muscle cells). Primary antibody: Anti-GAPDH Mouse mAb (6C5) (Cat. No. CB1001) (assay dependent of Primary Antibody). Detection: fluorescence.

Detection of GAPDH by immunoblotting. Samples: Tissue exctract from isolated human GAPDH (lane 1), human heart (lane 2), pig heart (lane 3), goat heart (lane 4), bovine heart (lane 5), dog heart (lane 6), mouse heart (lane 7), rat heart (lane 8), rabbit heart (lane 9), and duck heart (lane 10). Primary antibody: Anti-GAPDH Mouse mAb (6C5) (Cat. No. CB1001) (1 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.4.
Positive controlRat skeletal muscle
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Gagarin, D., et al. 2005. J. Mol. Cell. Card. 39, 453. Yang, Z., et al. 2007. J. Vasc. Res. 44, 483.
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see comments)
Immunoblotting (1-10 µg/ml; see application references)
Immunocytochemistry (see comments)
Application CommentsUseful as a loading or positive control since GAPDH is expressed in all cells. Also reported to work for ELISA and immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified, rabbit muscle
ImmunogenRabbit
Clone6C5
HostMouse
IsotypeIgG₁
Species Reactivity
  • Canine
  • Chicken
  • Fish
  • Frog
  • Human
  • Mouse
  • Porcine
  • Rabbit
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Highly Toxic
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CB1001-500UGCN 04055977228649

Documentation

Anti-GAPDH Mouse mAb (6C5) SDS

Title

Safety Data Sheet (SDS) 

Anti-GAPDH Mouse mAb (6C5) Certificates of Analysis

TitleLot Number
CB1001
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision30-November-2007 JSW
SynonymsAnti-Glyceraldehyde-3-Phosphate Dehydrogenase
ApplicationELISA (see comments)
Immunoblotting (1-10 µg/ml; see application references)
Immunocytochemistry (see comments)
Application Data
Detection of rat GAPDH by immunocytochemistry. Sample: A-10 cell line (rat aortic smooth muscle cells). Primary antibody: Anti-GAPDH Mouse mAb (6C5) (Cat. No. CB1001) (assay dependent of Primary Antibody). Detection: fluorescence.

Detection of GAPDH by immunoblotting. Samples: Tissue exctract from isolated human GAPDH (lane 1), human heart (lane 2), pig heart (lane 3), goat heart (lane 4), bovine heart (lane 5), dog heart (lane 6), mouse heart (lane 7), rat heart (lane 8), rabbit heart (lane 9), and duck heart (lane 10). Primary antibody: Anti-GAPDH Mouse mAb (6C5) (Cat. No. CB1001) (1 µg/ml). Detection: chemiluminescence.
DescriptionProtein A purified mouse monoclonal antibody. Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.
BackgroundGlyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key enzymes involved in glycolysis. GAPDH is comprised of four ~36 kDa subunits with a total molecular weight of ~146 kDa. Besides functioning as a glycolytic enzyme in cytoplasm, mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recently, findings have been made concerning the role of GAPDH in differentpathologies including prostate cancer progression, programmed neuronal cell death, and age-related neuronal diseases such as Alzheimer’s Disease and Huntington’s Disease. While GAPDH is constitutively expressed in almost all tissues at high levels, there are some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types.
HostMouse
Immunogen speciesRabbit
Immunogenpurified, rabbit muscle
Clone6C5
IsotypeIgG₁
Speciesnot bovine, canine, chicken, fish, frog, human, mouse, porcine, rabbit, rat, not yeast
Positive controlRat skeletal muscle
FormLiquid
FormulationIn PBS, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsUseful as a loading or positive control since GAPDH is expressed in all cells. Also reported to work for ELISA and immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Toxicity Highly Toxic
Application referencesImmunoblotting Gagarin, D., et al. 2005. J. Mol. Cell. Card. 39, 453. Yang, Z., et al. 2007. J. Vasc. Res. 44, 483.