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ABE2590-100UL Anti-H3K4me3Q5dopaminyl

Anti-H3K4me3Q5dopaminyl, Cat. No. ABE2590, is a rabbit polyclonal antibody that detects Histone H3.1 dopaminyated on glutamine 5 and is tested for use in Dot Blot, Peptide Inhibition Assay, and Western Blotting.

ABE2590-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABE2590-100UL
DescriptionAnti-H3K4me3Q5dopaminyl
Alternate Names
  • Dopaminylated Histone H3
  • H3Q5dopaminylated
Background InformationHistone H3.1t (UniProt: Q16695; also known as H3/t, H3t, H3/g) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. The H3.1 and H3.3 complexes also possess distinct histone chaperones, CAF-1 and HIRA, which play important role in mediating DNA-synthesis-dependent and -independent nucleosome assembly. Histone H 3.1 is a core component of nucleosome that is present only in mammals. It is expressed during S phase, then its expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It serves as the canonical histone, which is incorporated during DNA replication.
Histones are dynamic proteins that undergo multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone 3.1 is also shown to undergo serotonylation of glutamine at position 5 (Q5ser) in organisms that produce serotonin. Recently, it has been reported that Histone H3 can undergo dopaminylation ((H3Q5dop), which can potentiate the binding of adjacent histone post-translational modification interacting proteins and play a vital role in dopaminergic neuronal transcription. This rabbit polyclonal antibody specifically binds to Histone H3 dopaminylated on glutamine 5.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-H3K4me3Q5dopaminyl, Cat. No. ABE2590, is a rabbit polyclonal antibody that detects Histone H3.1 dopaminyated on glutamine 5 and is tested for use in Dot Blot, Peptide Inhibition Assay, and Western Blotting.
Key Applications
  • Dot Blot
  • Peptide Inhibition Assay
  • Western Blotting
Application NotesDot Blot Analysis: A 1:1,000 dilution from a representative lot detected H3K4me3Q5dopaminyl in H3(K4me3Q5dopaminyl) & H3 control peptide (Courtesy of Ian Maze Lab, Icahn School of Medicine at Mount Sinai, New York).

Western Blotting Analysis: A 1:200 dilution from a representative lot detected H3K4me3Q5dopaminyl in human and rat ventral tegmental area (Courtesy of Ian Maze Lab, Icahn School of Medicine at Mount Sinai, New York).

Peptide Inhibition Assay: Target band detection in H3K4me3Q5dopaminyl was prevented by preblocking of a representative lot with the immunogen peptide, but not with a control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H3 with trimethylated lysine 4 and dopaminylated on glutamine 5.
EpitopeN-terminus
Concentration0.03 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Histone H3 dopaminylated on glutamine 5.
IsotypeIgG
Species Reactivity
  • Rat
  • Human
Species Reactivity NoteHuman, Rat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST3H3
  • H3FT
Purification MethodAffinity Purified
UniProt Number
Molecular Weight15.51 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Dot Blot in H3(K4me3Q5dopaminyl) & H3 control peptide.



Western Blotting Analysis: A 1:500 dilution of this antibody detected H3K4me3Q5dopaminyl, but not the H3 control peptide.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE2590-100UL 04061842196265

Documentation

Anti-H3K4me3Q5dopaminyl SDS

Title

Safety Data Sheet (SDS) 

Anti-H3K4me3Q5dopaminyl Certificates of Analysis

TitleLot Number
Anti-H3K4me3Q5dopaminyl - 3713358 3713358
Anti-H3K4me3Q5dopaminyl - 3731629 3731629
Anti-H3K4me3Q5dopaminyl - 3733752 3733752
Anti-H3K4me3Q5dopaminyl - 3761798 3761798
Anti-H3K4me3Q5dopaminyl - 3849520 3849520
Anti-H3K4me3Q5dopaminyl - 3858258 3858258
Anti-H3K4me3Q5dopaminyl - 3923694 3923694
Anti-H3K4me3Q5dopaminyl - 3927998 3927998
Anti-H3K4me3Q5dopaminyl - 4020562 4020562
Anti-H3K4me3Q5dopaminyl - 4040798 4040798
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