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ABS810 Anti-IKK beta Antibody

This Anti-IKK beta Antibody is validated for use in Western Blotting_x000D_ and Immunocytochemistry for the detection of IKK beta.

Anti-IKK beta Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: IKBKB, IKKB UniProt Number: O14920
ABS810
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS810
Replaces04-366
DescriptionAnti-IKK beta Antibody
Alternate Names
  • Inhibitor of nuclear factor kappa-B kinase subunit beta
  • I-kappa-B-kinase beta
  • IKK-B
  • IKK-beta
  • IkBKB
Background InformationIKK-beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquitination. IKK-beta has been shown to activate NF-kB and phosphorylate IKB-alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK-beta is essential for activation of IKK by TNF and IL1. Once activated, IKK-beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK-beta activity can also be regulated by MEKK-1.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing PBS (pH 7.2) with 0.02% sodium azide.
Quality LevelMQ300
Applications
ApplicationThis Anti-IKK beta Antibody is validated for use in Western Blotting_x000D_ and Immunocytochemistry for the detection of IKK beta.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis (ICC): 10 μg/mL from a representative lot detected IKK beta in HeLa cells.
Biological Information
ImmunogenIKK beta antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IKK beta (Genbank accession NoO14920), which differs from corresponding murine sequence by one amino acid.
EpitopeNear C-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Gene Symbol
  • IKBKB
  • IKKB
Purification MethodAntigen Affinity Purified
UniProt Number
Molecular Weight~88 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat whole cell lysate.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected IKK beta in Jurkat whole cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS810 04055977174687