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ABE195 Anti-JMJD1A Antibody

Anti-JMJD1A Antibody is a Rabbit Polyclonal Antibody for detection of JMJD1A also known as lysine (K)-specific demethylase 3A, testis-specific protein A
JMJD1A, JMJD1 & has been validated in WB.

Anti-JMJD1A Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: JHDM2A, TSGA, JHMD2A, JMJD1A, JMJD1, KDM3A UniProt Number: Q9Y4C1
ABE195
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbSerumPolyclonal Antibody
Description
Catalogue NumberABE195
DescriptionAnti-JMJD1A Antibody
Alternate Names
  • JMJD
  • lysine (K)-specific demethylase 3A
  • testis-specific protein A
  • jumonji domain containing 1A
  • jumonji C domain-containing histone demethylase 2A
  • jumonji domain containing 1
  • Jumonji domain-containing protein 1A
  • JmjC domain-containing histone demethylation protein 2A
  • lysine-specific demethylase 3A
    JMJD1A
  • JMJD1
Background InformationJMJD1A (jumonji domain-containing 1a), also known as JHDM2A is member of the jumonji (jmj) domain containing gene family of proteins. This protein functions as a histone demethylase that demethylates mono and dimethyl lysine 9 of histone H3. Consequently, this protein plays a role in the regulation of chromatin structure and influences transcriptional activation and suppression. Loss of function of JMJD1A leads to embryonic stem cell differentiation. JMJD1A has also been implicated in smooth muscle cell-specific transcription, hypoxic gene expression and tumor growth.
References
Product Information
FormatSerum
Control
  • HeLa nuclear extract
PresentationRabbit polyclonal serum containing 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-JMJD1A Antibody is a Rabbit Polyclonal Antibody for detection of JMJD1A also known as lysine (K)-specific demethylase 3A, testis-specific protein A
JMJD1A, JMJD1 & has been validated in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human JMJD1A.
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq].
Gene Symbol
  • JHDM2A
  • TSGA
  • JHMD2A
  • JMJD1A
  • JMJD1
  • KDM3A
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.

COFACTOR: Binds 1 Fe2+ ion per subunit.

SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note: Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity).

DOMAIN: The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.

Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors (By similarity).

SEQUENCE SIMILARITIES: Belongs to the JHDM2 histone demethylase family.

Contains 1 JmjC domain.

SEQUENCE CAUTION: The sequence BAA34462.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
Molecular Weight ~147 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 1:1,000 dilution of this antibody detected JMJD1A on 10 µg of HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE195 04053252415333