Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MAB1924-C-25UG Anti-Laminin alpha5 Antibody, clone 4C7 (Ascites Free)

This mouse monoclonal Anti-Laminin alpha5, clone 4C7 (Ascites Free), Cat. No. MAB1924-C-25UG is validated for use in Immunoprecipitation Immunofluorescence, Immunohistochemistry, ELISA and Neutralizing, for the detection of Laminin alpha 5.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: LAMA5, KIAA0533, KIAA1907 UniProt Number: O15230
MAB1924-C-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIP, IF, IHC, ELISA, NEUTMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB1924-C-25UG
DescriptionAnti-Laminin alpha5 Antibody, clone 4C7 (Ascites Free)
Alternate Names
  • Laminin-10 subunit alpha
  • Laminin-11 subunit alpha
  • Laminin-15 subunit alpha
Background InformationLaminin subunit alpha-5 (UniProt: O15230; also known as Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha) is encoded by the LAMA5 (also known as KIAA0533, KIAA1907) gene (Gene ID: 3911) in human. Laminins are a family of heterotrimeric extracellular glycoproteins consisting of alpha, beta, and gamma chains. Laminins mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Thus far five alpha, three beta, and three gamma subunits are known, which assemble into at least 15 different laminin molecules. The alpha, beta, and gamma chains are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-5 is a subunit of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). It contains 22 laminin EGF-like domains, 5 5 laminin G-like domains, 1 laminin IV type A domain, and 1 laminin N-terminal domain. Laminin 5-alpha is expressed in heart, lung, kidney, muscle, pancreas, retina, and placenta. Very little or no expression is observed in brain and liver.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal Anti-Laminin alpha5, clone 4C7 (Ascites Free), Cat. No. MAB1924-C-25UG is validated for use in Immunoprecipitation Immunofluorescence, Immunohistochemistry, ELISA and Neutralizing, for the detection of Laminin alpha 5.
Key Applications
  • Immunoprecipitation
  • Immunofluorescence
  • Immunohistochemistry
  • ELISA
  • Neutralizing
Application NotesImmunohistochemistry Analysis: A representative lot detected Laminin alpha 5 in immunostained basement membranes in O.T.C.-embedded fetal and adult human lung cryosections (Pierce, R.A., et al. (2000). Am. J. Respir. Cell Mol. Biol. 23(6):742-747).

Neutralizing Analysis: A representative lot detected Laminin alpha 5 in adhesion of sickle cell patients (Hb SS) red blood cells (RBCs) to human placental laminin-coated surface (Lee, S.P., et al. (1998). Blood. 92(8):2951-2958).

ELISA Analysis: A representative lot detected Laminin alpha 5 in recombinant human (rh) Laminin-10 ( 5 1 1) and Laminin-11 ( 5 2 1), but not Laminin-8 ( 4 1 1) (Wondimu, Z., et al. (2013). PLoS One. 8(1):e53648).

Immunofluorescence Analysis: A representative lot detected Laminin alpha 5 in immunoreactivity in human skin and kidney cryosections (Wondimu, Z., et al. (2013). PLoS One. 8(1):e53648).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Laminin alpha 5 in Laminin-10 ( 5 1 1) from A549 culture medium (Wondimu, Z., et al. (2013). PLoS One. 8(1):e53648).
Biological Information
ImmunogenFull length purified human laminin alpha 5.
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 4C7 reacts with the globular domain of the alpha5 chain of intact laminin and blocks the neurite stimulating activity of laminin.
IsotypeIgG2a
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • LAMA5
  • KIAA0533
  • KIAA1907
Purification MethodProtein G purified
UniProt Number
Molecular Weight260 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunoprecipitation in A431 cell lysate.
Immunoprecipitation Analysis: 10 µg of this antibody i mmunoprecipitated Laminin alpha 5 in 500 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB1924-C-25UG 04054839447952