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MABF1971-25UL Anti-NKR-P1B (CD161b) Antibody, clone Stok27

This rat monoclonal Anti-NKR-P1B (CD161b), clone Stok27 Antibody, Cat. No. MABF1971-25UL is validated for use in Flow Cytometry and Immunoprecipitation, for the detection of NKR-P1B.

Anti-NKR-P1B (CD161b) Antibody, clone Stok27 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Klrb1b, Nkrp1b, Nkrp1c UniProt Number: Q5NKN4
MABF1971-25UL
25 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RFC, IPRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF1971-25UL
DescriptionAnti-NKR-P1B (CD161b) Antibody, clone Stok27
Alternate Names
  • Killer cell lectin-like receptor subfamily B member 1B allele B
  • CD161 antigen-like family member B
  • Immunoreceptor NKR-P1C
  • Natural killer cell surface protein NKR-P1B allele WAG/PVG/BS
  • Natural killer lymphocyte receptor P1B
  • CD161b
Background InformationKiller cell lectin-like receptor subfamily B member 1B allele B (UniProt: Q5NKN4; also known as CD161 antigen-like family member B, Immunoreceptor NKR-P1C, Natural killer cell surface protein NKR-P1B allele WAG/PVG/BS, Natural killer lymphocyte receptor P1B, CD161b) is encoded by the Klrb1b (also known as Nkrp1b, Nkrp1c) gene (Gene ID: 678513) in rat. NKR-P1B is a single-pass type II membrane protein that is expressed in a subset of natural killer cells. It consists of a cytoplasmic domain (aa 1-45), a helical domain (aa 46-66), and an extracellular domain (aa 67-223). In contrast to NKR-P1C, which functions as an activating receptor, NKR-P1B inhibits NK cell activation. NKR-P1b serves as a receptor for CLEC2D/OCIL and ligand binding contributes to inhibition of cytotoxic natural killer cells. It may also mediate MHC class I-independent 'missing-self' recognition of allografts, tumor cells, and virus-infected cells. The inhibitory NKR-P1B receptor identifies rat splenic NK cells that are low in Ly49 receptors, but are enriched in CD94/NKG2 receptors. (Ref.: Inngjerdingen, M et al. (2012). J. Immunol. 188(6); 2499-2508; Carlyle, JR et al. (1999). J. Immunol. 162(10); 5917-5923).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationThis rat monoclonal Anti-NKR-P1B (CD161b), clone Stok27 Antibody, Cat. No. MABF1971-25UL is validated for use in Flow Cytometry and Immunoprecipitation, for the detection of NKR-P1B.
Key Applications
  • Flow Cytometry
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: A representative lot detected NKR-P1B (CD161b) in Immunoprecipitation applications (Kveberg, L., et. al. (2006). J Immunol. 176(7):4133-40).

Flow Cytometry Analysis: A representative lot detected NKR-P1B (CD161b) in Flow Cytometry applications (Kveberg, L., et. al. (2006). J Immunol. 176(7):4133-40; Smelt, M.J., et. al. (2014). Cell Transplant. 23(11):1381-94).
Biological Information
ImmunogenIL-2-activated NK cells from PVG.1N (RT1n) rats.
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone Stok27 is a rat monoclonal antibody that detects Killer cell lectin-like receptor subfamily B member 1B allele B (NKR-P1B) in rat. It targets an epitope within the extracellular domain.
IsotypeIgG2a
Species Reactivity
  • Rat
Species Reactivity NoteRat.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Klrb1b
  • Nkrp1b
  • Nkrp1c
Purification MethodProtein G purified
UniProt Number
Molecular Weight25 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in Rnk16 (Rat NK) cells stably transfected with Stok27 (NKR-1B) antigen.

Flow Cytometry Analysis: 1 µg of this antibody detected NKR-P1B (CD161b) in Rnk16 (Rat NK) cells stably transfected with Stok27 (NKR-1B) antigen.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF1971-25UL 04054839456169