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ST1535 Anti-NQO1 Rabbit pAb

This Anti-NQO1 Rabbit pAb is validated for use in Immunoblotting for the detection of NQO1.

Anti-NQO1 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-NADPH Dehydrogenase Quinone 1

ST1535
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
ST1535-100UGCN Glass bottle 100 μg
Description
OverviewRecognizes the ~30 kDa NQO1 protein in human stomach tissue, mouse brain tissue, and HepG2 cells.
Catalogue NumberST1535
Brand Family Calbiochem®
SynonymsAnti-NADPH Dehydrogenase Quinone 1
Application Data
Detection of human NQO1 by immunoblotting. Sample: Whole tissue extract from human stomach. Primary antibody: Anti-NQO1 Rabbit pAb (Cat. No. ST1535) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse NQO1 by immunoblotting. Sample: Whole tissue extract from mouse brain. Primary antibody: Anti-NQO1 Rabbit pAb (Cat. No. ST1535) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human NQO1 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-NQO1 Rabbit pAb (Cat. No. ST1535) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlHuman stomach tissue, Mouse brain tissue, HepG2 cells
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1-5 µg/ml)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant human NQO1 (aa 1-274)
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1535-100UGCN 04055977224238

Documentation

Anti-NQO1 Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-November-2010 RFH
SynonymsAnti-NADPH Dehydrogenase Quinone 1
ApplicationImmunoblotting (1-5 µg/ml)
Application Data
Detection of human NQO1 by immunoblotting. Sample: Whole tissue extract from human stomach. Primary antibody: Anti-NQO1 Rabbit pAb (Cat. No. ST1535) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse NQO1 by immunoblotting. Sample: Whole tissue extract from mouse brain. Primary antibody: Anti-NQO1 Rabbit pAb (Cat. No. ST1535) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human NQO1 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-NQO1 Rabbit pAb (Cat. No. ST1535) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified rabbit polyclonal antibody. Recognizes the ~30 kDa NQO1 protein.
BackgroundNQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD).
HostRabbit
Immunogen speciesHuman
ImmunogenFull-length, recombinant human NQO1 (aa 1-274)
IsotypeIgG
Specieshuman, mouse
Positive controlHuman stomach tissue, Mouse brain tissue, HepG2 cells
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
CommentsVariables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review