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OP180 Anti-Noxa Mouse mAb (114C307)

This Anti-Noxa Mouse mAb (114C307) is validated for use in Immunoblotting for the detection of Noxa.

Anti-Noxa Mouse mAb (114C307) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP180
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP180-100UGCN Plastic ampoule 100 μg
Description
OverviewRecognizes the ~11 kDa Noxa protein in RL-7 cells.
Catalogue NumberOP180
Brand Family Calbiochem®
Application Data
Detection of human Noxa by immunoblotting. Sample: Whole cell lysate from RL-7 cells. Primary antibody: Anti-Noxa Mouse mAb (114C307) (Cat. No. OP180) (2 µg/ml). Detection: chemiluminescence.
References
ReferencesOda, E., et al. 2000. Science 288, 1053.
Product Information
FormLiquid
FormulationIn PBS, 0.05% BSA.
Negative controlNormal mouse serum
Positive controlRL-7 cell lysate
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1-2 µg/ml, chemiluminescence)
Application CommentsAntibody should be titrated for optimal results in individual system.
Biological Information
Immunogena recombinant protein consisting of human Noxa fused to GST
ImmunogenHuman
Clone114C307
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C;)
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP180-100UGCN 04055977208849

Documentation

Anti-Noxa Mouse mAb (114C307) SDS

Title

Safety Data Sheet (SDS) 

Anti-Noxa Mouse mAb (114C307) Certificates of Analysis

TitleLot Number
OP180

References

Reference overview
Oda, E., et al. 2000. Science 288, 1053.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure

Citations

Title
  • Yolanda Fernandez, et al. (2006) Chemical Blockage of the Proteasome Inhibitory Function of Bortezomib: Impact on Tumor Cell Death. Journal of Biological Chemistry 281, 1107-1118.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision08-October-2007 JSW
    ApplicationImmunoblotting (1-2 µg/ml, chemiluminescence)
    Application Data
    Detection of human Noxa by immunoblotting. Sample: Whole cell lysate from RL-7 cells. Primary antibody: Anti-Noxa Mouse mAb (114C307) (Cat. No. OP180) (2 µg/ml). Detection: chemiluminescence.
    DescriptionPurified mouse monoclonal antibody. Recognizes the ~11 kDa Noxa protein.
    BackgroundNoxa, a BH3 member of the Bcl-2 family, has recently been identified in human and mouse. Expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa occurs in normal thymocytes, but does not occur in p53-deficient thymocytes. Coimmunoprecipitation data suggests that Noxa may interact with other members of the Bcl-2 family, including Bcl-XL and Mcl-1. Data has also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treating cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis, and this suggests that Noxa may be a mediator of p53-dependent apoptosis.
    HostMouse
    Immunogen speciesHuman
    Immunogena recombinant protein consisting of human Noxa fused to GST
    Clone114C307
    IsotypeIgG₁
    Specieshuman
    Positive controlRL-7 cell lysate
    Negative controlNormal mouse serum
    FormLiquid
    FormulationIn PBS, 0.05% BSA.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsAntibody should be titrated for optimal results in individual system.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C;)
    Toxicity Standard Handling
    ReferencesOda, E., et al. 2000. Science 288, 1053.
    Citation
  • Yolanda Fernandez, et al. (2006) Chemical Blockage of the Proteasome Inhibitory Function of Bortezomib: Impact on Tumor Cell Death. Journal of Biological Chemistry 281, 1107-1118.
    		•