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MABE1040 Anti-Nup98 Antibody, clone 2H10

Anti-Nup98 Antibody, clone 2H10 is an antibody against Nup98 for use in Western Blotting and Immunocytochemistry.

Anti-Nup98 Antibody, clone 2H10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: NUP98, ADAR2 UniProt Number: P52948
MABE1040
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICCRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1040
DescriptionAnti-Nup98 Antibody, clone 2H10
Alternate Names
  • Nuclear pore complex protein Nup98-Nup96
  • Nuclear pore complex protein Nup98
  • 98 kDa nucleoporin
  • Nucleoporin Nup98
  • Nup98
  • Nuclear pore complex protein Nup96
  • 96 kDa nucleoporin
  • Nucleoporin Nup96
  • Nup96
  • Nup98
Background InformationThe human gene NUP98 (Gene ID 4928; Also known as ADAR2, ADIR2, NUP96, NUP196) encodes the nuclear pore complex protein Nup98-Nup96 (UniProt P52948; also known as GLFG-repeat containing nucleoporin, Nup98-Nup96), which is posttranslationally autocleaved to form Nup98 and Nup96. Cleavage of the Nup98-96 linkage enhances the interaction between Nup98 and Nup96 and promotes localization of the two proteins to the nuclear pore complex (NPC). An alternative splice event generates a mRNA variant (isoform 3; also known as Nup98-specific 1), which expresses only the Nup98 portion of the Nup98-96 mRNA and allows the independent production of Nup98. Unlike Nup96, Nup98 is known to exist in both NPC-bound and non-NPC-associated pool, where it may participate in gene transcription regulations. Nup98 is reported to interact with histone-modifying enzymes CBP/p300 and histone deacetylases (HDACs) through its glycine-leucine-phenylalanine-glycine (GLFG) repeats. NUP98 gene is also involved in chromosomal translocations, resulting in Nup98 translocation fusions identified as potent transcription factors that induce acute myeloid leukemia (AML) with a particularly poor patient prognosis.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2cκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Nup98 Antibody, clone 2H10 is an antibody against Nup98 for use in Western Blotting and Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A representative lot detected Nup98 in Rat neurons.
Immunocytochemistry Analysis: A representative lot detected Nup98 nuclear localization in HeLa cells (Fukuhara, T., et al. (2005) Hybridoma (Larchmt). 24(5):244-247).
Immunocytochemistry Analysis: A representative lot detected nuclear localization Nup98 in NIH/3T3 cells (Oka, M., et al. (2010) Mol Biol Cell. 21(11):1885-1896).
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human Nup98 near the N-terminus.
EpitopeNear N-terminus
Clone2H10
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityReacts with Nup98-Nup96 precursor, nucleoprotein Nup98, but not nucleoprotein Nup96.
IsotypeIgG2cκ
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NUP98
  • ADAR2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~100 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Nup98 in 10 µg of HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1040 04055977170696

Documentation

Anti-Nup98 Antibody, clone 2H10 SDS

Title

Safety Data Sheet (SDS) 

Anti-Nup98 Antibody, clone 2H10 Certificates of Analysis

TitleLot Number
Anti-Nup98, clone 2H10 - 3326074 3326074
Anti-Nup98, clone 2H10 - 3449073 3449073
Anti-Nup98, clone 2H10 - 3911629 3911629
Anti-Nup98, clone 2H10 -Q2573210 Q2573210

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.