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MABN1797 Anti-Prdx-6 Antibody, clone TAG-2A12

Anti-Prdx-6 Antibody, clone TAG-2A12, Cat. No. MABN1797, is a highly specific mouse monoclonal antibody that targets Prdx-6 and has been tested in Flow Cytometry, Immunofluorescence, Immunocytochemistry and Immunoprecipitation, and Western Blotting.

MABN1797
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABN1797
DescriptionAnti-Prdx-6 Antibody, clone TAG-2A12
Alternate Names
  • Peroxiredoxin-6
  • EC: 1.11.1.15
  • 1-Cys peroxiredoxin
  • 1-Cys PRX
  • 24 kDa protein
  • Acidic calcium-independent phospholipase A2
  • aiPLA2
  • Antioxidant protein 2
  • Liver 2D page spot 40
  • Non-selenium glutathione peroxidase
  • NSGPx
Background InformationPeroxiredoxin-6 (UniProt: P30041; also known as EC: 1.11.1.15, 1-Cys peroxiredoxin, 1-Cys PRX, 24 kDa protein, Acidic calcium-independent phospholipase A2, aiPLA2, Antioxidant protein 2, Liver 2D page spot 40, Non-selenium glutathione peroxidase, NSGPx) is encoded by the PRDX6 (also known as AOP2, KIAA0106) gene (Gene ID: 9588) in human. Peroxiredoxin-6 is a cytoplasmic protein that is involved in redox regulation of the cell. It reduces hydrogen peroxide and short chain organic fatty acid, and phospholipid hydroperoxides. It is the only mammalian 1-Cys member of the Prdx family with bifunctional - glutathione peroxidase and phospholipase A2 activities. Peroxiredoxin-6 shares several structural and functional properties with other members of the Prdx family, however, it has only one conserved cysteine residue and its thioredoxin does not participate in the catalytic cycle. Peroxiredoxin-6 is expressed in higher levels in epithelium such as apical regions of respiratory epithelium and skin epidermis and within the lung, it is expressed at relatively high levels within the type II alveolar epithelial cells and bronchiolar Clara cells. Lower levels of expression are reported in alveolar macrophages and microvascular endothelium. Human peroxiredoxin-6 shares 87% sequence identity with mouse, rat, and bovine.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Prdx-6 Antibody, clone TAG-2A12, Cat. No. MABN1797, is a highly specific mouse monoclonal antibody that targets Prdx-6 and has been tested in Flow Cytometry, Immunofluorescence, Immunocytochemistry and Immunoprecipitation, and Western Blotting.
Key Applications
  • Flow Cytometry
  • Immunoprecipitation
  • Immunofluorescence
  • Immunocytochemistry
  • Western Blotting
Application NotesImmunoprecipitation Analysis: A representative lot detected Prdx-6 in Immunoprecipitation applications (Ding, V., et al. (2014). MAbs. 6(6):1439-52).

Immunocytochemistry Analysis: A representative lot detected Prdx-6 in Immunocytochemistry applications (Ding, V., et al. (2014). MAbs. 6(6):1439-52).

Flow Cytometry Analysis: A representative lot detected Prdx-6 in Flow Cytometry applications (Ding, V., et al. (2014). MAbs. 6(6):1439-52).

Immunofluorescence Analysis: A representative lot detected Prdx-6 in Immunofluorescence applications (Ding, V., et al. (2014). MAbs. 6(6):1439-52).

Western Blotting Analysis: A representative lot detected Prdx-6 in Western Blotting applications (Ding, V., et al. (2014). MAbs. 6(6):1439-52).

Flow Cytometry Analysis: A representative lot detected Prdx-6 in human corneal endothelial cells from a donor. (Courtesy of Dr. Vanessa Ding, Ph.D. and Dr. Andre Choo, Ph.D, BTI, Singapore).
Biological Information
ImmunogenCorneal endothelial cells (hCENC) isolated from corneas of human cadavers and immunized with monophosphoryl-lipid A trehalose dicorynomycolate (MPLCTDM) adjuvant.
CloneTAG-2A12
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone TAG-2A12 detects Peroxiredoxin-6 in human cells.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PRDX6
  • AOP2
  • KIAA0106
Purification MethodProtein G purified
UniProt Number
Molecular Weight~28 kDa observed; 25.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIdentity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be human IgG1 .
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN1797 04054839233197

Documentation

Anti-Prdx-6 Antibody, clone TAG-2A12 Certificates of Analysis

TitleLot Number
Anti-Prdx-6, clone TAG-2A12 - 3490049 3490049
Anti-Prdx-6, clone TAG-2A12 - 3756208 3756208
Anti-Prdx-6, clone TAG-2A12 - 3862810 3862810
Anti-Prdx-6, clone TAG-2A12 - 3918805 3918805
Anti-Prdx-6, clone TAG-2A12 - 3958816 3958816
Anti-Prdx-6, clone TAG-2A12 - 3959202 3959202
Anti-Prdx-6, clone TAG-2A12 - 4088389 4088389
Anti-Prdx-6, clone TAG-2A12 - 4101920 4101920
Anti-Prdx-6, clone TAG-2A12 - 4122591 4122591
Anti-Prdx-6, clone TAG-2A12 - 4201322 4201322
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