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MABE1167 Anti-SPO11 Antibody, clone 180

Anti-SPO11, clone 180, Cat. No. MABE1167, is a highly specific mouse monoclonal antibody that targets SPO11 and has been tested in and Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Anti-SPO11 Antibody, clone 180 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Spo11 UniProt Number: Q9WTK8
MABE1167
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWB, IP, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1167
DescriptionAnti-SPO11 Antibody, clone 180
Alternate Names
  • Meiotic recombination protein SPO11
Background InformationMeiotic recombination protein SPO11 (UniProt Q9WTK8) is encoded by the Spo11 gene (Gene ID 26972) in murine species. SPO11 is a widely conserved protein that is involved in meiotic recombination and mediates DNA cleavage that forms the double-strand breaks that initiate meiotic recombination. Active site of SPO11 contains a tyrosine, which ligates and dissociates with DNA to promote break formation. One SPO11 protein is involved per strand of DNA, hence, two SPO11 proteins are involved in each double stranded break event. High levels of SPO11 are found only in the testis where expression is restricted primarily to meiotic germ cells and it is not expressed in spermatogonia. Very low levels of SPO11 have been reported in thymus, brain, and oocytes of embryonic ovary, but it is not detected in adult ovary. Mouse and human SPO11 exhibit about 82% sequence homology. Ref.: Keeney, S., et al. (1997). Cell 375-384.
References
Product Information
FormatPurified
PresentationPurified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-SPO11, clone 180, Cat. No. MABE1167, is a highly specific mouse monoclonal antibody that targets SPO11 and has been tested in and Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
Application NotesImmunoprecipitation Analysis: 3 µg from a representative lot immunoprecipitated SPO11alpha and SPO11beta from wild-type, but not Spo11-knockout mouse testes lysates (Courtesy of the Keeney Lab, MSKCC, New York, U.S.A.).

Western Blotting Analysis: A representative lot detected SPO11alpha and SPO11beta in immunoprecipitate from wild-type, but not SPO-knockout, mouse testes using the same antibody (Courtesy of the Keeney Lab, MSKCC, New York, U.S.A.).

Immunocytochemistry Analysis: A representative lot immunostained SPO11 in meiotic prophase I leptotene cells by fluorescence immunocytochemistry staining of chromosome spreads from isolated mouse spermatocytes hypotonically swollen, fixed with 1% formaldehyde and permeabilized with 0.1% Triton X-100 (Alpatov, R., et al. (2014). Cell. 157(4):869-881).

Immunoprecipitation Analysis: A representative lot immunoprecipitated SPO11alpha and SPO11beta as well as SPO11-oligo complexes from mouse testes lysates. Much elevated and greatly reduced SPO11-oligo complexes were found in Atm-/- and Dmc-/- testes IP, respectively, when comparing to wild-type testes IP (Lange, J., et al. (2011). Nature. 479(7372):237-240).

Western Blotting Analysis: Representative lots detected SPO11alpha and SPO11beta in immunoprecipitates obtained with the same antibody from mouse testes. Much reduced SPO11 was found in Atm-/- and Dmc-/- testes IPs due to meiotic arrest at prophase I (Biswas, U., et al. (2013). PLoS Genet. 9(12):e1003985; Lange, J., et al. (2011). Nature. 479(7372):237-240).
Biological Information
ImmunogenRecombinant mouse SPO11 with N-terminal S-tag and C-terminal His-tag.
Clone180
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 180 detected SPO11alpha and SPO11beta in wild-type, but not Spo11-knockout mouse testes lysates by IP-Western blotting.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Spo11
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~44/40 kDa (SPO11beta/SPO11alpha) observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIdentity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2aκ.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1167 04054839105517