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MAB4301-25UG Anti-SSEA-1, clone MC-480

Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480 detects level of Stage-Specific Embryonic Antigen-1 & has been published & validated for use in FC, IF, IH & IP.

Anti-SSEA-1, clone MC-480 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation, Neural Stem Cells Gene Symbol: FUT4, ELFT, FCT3A, Fuc-TIV, 1,3fucosyltransferase, FUC-TIV, FucT-IV, CD15, Alpha-, EC 2.4.1.- UniProt Number: P22083
MAB4301-25UG
25 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RFC, IF, IHC, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB4301-25UG
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-SSEA-1, clone MC-480
Alternate Names
  • SSEA-1
  • CD15
  • Lewis X
  • 3-FAL
Background InformationSSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
References
Product Information
FormatPurified
Control
  • P19 mouse embryonic carcinoma cells
PresentationPurified  Potassium Phosphate, 0.3M NaCl, pH 8.0 with 0.05% Sodium Azide mouse monoclonal IgM in buffer containing 0.05M 
Quality LevelMQ100
Applications
ApplicationAnti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480 detects level of Stage-Specific Embryonic Antigen-1 & has been published & validated for use in FC, IF, IH & IP.
Key Applications
  • Flow Cytometry
  • Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
Application NotesImmunohistochemistry:
A previous lot of this antibody was tested on IH.

Immunoprecipitation:
A previous lot of this antibody was tested on IP.

Immunofluorescence:
A previous lot of this antibody was tested on IF. 4% PFA fixed, 5-10 minutes room temperature. No detergent is required as epitope is external.

Flow Cytometry:
A starting range of 10-20 µg/mL is suggested.

Positive mouse EC lines include:
F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25.
Lines : PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147 were negative.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenF9 tetracarcinoma stem cells (X-irradiated).
CloneMC-480
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityThis antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
IsotypeIgM
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryFUT4 catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). It has been detected in human embryos (5-10 weeks) suggesting a role in development.
Gene Symbol
  • FUT4
  • ELFT
  • FCT3A
  • Fuc-TIV
  • 1,3fucosyltransferase
  • FUC-TIV
  • FucT-IV
  • CD15
  • Alpha-
  • EC 2.4.1.-
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P22083 # May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
SIZE: 405 amino acids; 45570 Da
SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
SIMILARITY: SwissProt: P22083 ## Belongs to the glycosyltransferase 10 family.
Stem Cell Type
  • Human Embryonic Stem Cells
  • Mouse Embryonic Stem Cells
  • Neural Stem Cells
  • Induced Pluripotent Stem Cells
Molecular Weight220 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceTested
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB4301-25UG 04054839350405