Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

DR1075 Anti-TARDBP Mouse mAb (2E2-D3)

This Anti-TARDBP Mouse mAb (2E2-D3) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TARDBP.

Anti-TARDBP Mouse mAb (2E2-D3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-TAR DNA Binding Protein

DR1075
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
DR1075-100UGCN 100 μg
Description
OverviewRecognizes the ~45 kDa TARDBP protein in A431 and HeLa cells and human leiomyosarcoma tissue.
Catalogue NumberDR1075
Brand Family Calbiochem®
SynonymsAnti-TAR DNA Binding Protein
Application Data
Detection of human TARDBP by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-TARDBP Mouse mAb (2E2-D3) (Cat. No. DR1075) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human TARDBP by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human leiomyosarcoma tissue. Primary antibody: Anti-TARDBP Mouse mAb (2E2-D3) (Cat. No. DR1075) (5 µg/ml). Detection: colorimetric (DAB).

Detection of human TARDBP by immunocytochemistry. Sample: HeLa cells fixed in paraformaldahyde. Primary antibody: Anti-TARDBP Mouse mAb (2E2-D3) (Cat. No. DR1075) (5 µg/ml). Detection: fluorescence.
References
ReferencesIlieva, E,V., et al. 2010. Free Radic. Biol. Med. 48, 1302.
Wils H, et al. 2010. Proc. Natl. Acad. Sci. U S A 107 3858.
Foulds, P,G., et al. 2009. Acta Neuropathol. 118 647.
Product Information
FormLiquid
FormulationIn PBS, pH 7.7.
Negative control293T cells
Positive controlA431 cells, Human leiomyosarcoma tissue, HeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesImmunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (5 µg/ml)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant human TARDBP (aa 1-261), expressed as a GST fusion protein.
Clone2E2-D3
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1075-100UGCN 04055977226058

Documentation

Anti-TARDBP Mouse mAb (2E2-D3) SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Ilieva, E,V., et al. 2010. Free Radic. Biol. Med. 48, 1302.
Wils H, et al. 2010. Proc. Natl. Acad. Sci. U S A 107 3858.
Foulds, P,G., et al. 2009. Acta Neuropathol. 118 647.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-November-2010 RFH
SynonymsAnti-TAR DNA Binding Protein
ApplicationImmunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (5 µg/ml)
Application Data
Detection of human TARDBP by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-TARDBP Mouse mAb (2E2-D3) (Cat. No. DR1075) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human TARDBP by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human leiomyosarcoma tissue. Primary antibody: Anti-TARDBP Mouse mAb (2E2-D3) (Cat. No. DR1075) (5 µg/ml). Detection: colorimetric (DAB).

Detection of human TARDBP by immunocytochemistry. Sample: HeLa cells fixed in paraformaldahyde. Primary antibody: Anti-TARDBP Mouse mAb (2E2-D3) (Cat. No. DR1075) (5 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~45 kDa TARDBP protein.
BackgroundHIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. TARDBP is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription.
HostMouse
ImmunogenFull-length, recombinant human TARDBP (aa 1-261), expressed as a GST fusion protein.
Clone2E2-D3
IsotypeIgG₁
Specieshuman
Positive controlA431 cells, Human leiomyosarcoma tissue, HeLa cells
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.7.
PreservativeNone
CommentsVariables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesIlieva, E,V., et al. 2010. Free Radic. Biol. Med. 48, 1302.
Wils H, et al. 2010. Proc. Natl. Acad. Sci. U S A 107 3858.
Foulds, P,G., et al. 2009. Acta Neuropathol. 118 647.