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MABN1537 Anti-TDO2 Antibody, clone 7A6.1

Anti-TDO2, clone 7A6.1, Cat. No. MABN1537, is a highly specific mouse monoclonal antibody that targets TDO2, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-TDO2 Antibody, clone 7A6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: TDO2, TDO UniProt Number: P48775
MABN1537
200 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1537
DescriptionAnti-TDO2 Antibody, clone 7A6.1
Alternate Names
  • Tryptophan 2
  • 3-dioxygenase
  • TDO
  • TO
  • TRPO
  • Tryptamin 2
  • 3-dioxygenase
  • Tryptophan oxygenase
  • Tryptophan pyrrolase
  • Tryptophanase
Background InformationTryptophan 2,3-dioxygenase (EC 1.13.11.11; UniProt P48775; also known as TDO, TO, TRPO, Tryptamin 2,3-dioxygenase, Tryptophan oxygenase, Tryptophan pyrrolase, Tryptophanase) is encoded by the TDO2 (also known as TDO) gene (Gene ID 6999) in human. TDO2 is a cytosolic heme-containing enzyme that exists as a homotetramer, where the N terminal residues of each monomer form part of the substrate binding site in an adjacent monomer. Tryptophan 2,3-dioxygenase (TDO2) catalyzes the first step of the kynurenine pathway, namely the conversion of tryptophan to kynurenine (Kyn), which can also be catalyzed by indoleamine 2,3-dioxygenase 1 (IDO1) or IDO2. IDO1 is expressed in tissues throughout the body, while TDO2 is predominantly expressed in the liver. Altered tryptophan metabolism and increased secretion of tryptophan metabolites are found in solid tumors, including in breast cancer. TDO2-mediated Kyn production in triple-negative breast cancer (TNBC) cells is reported to activate aryl hydrocarbon receptor (AhR), an endogenous kynurenine receptor. Inhibiting TDO2 or AhR via pharmacologic or genetic means is reported to result in increased sensitivity to anoikis, as well as decreased proliferation, migration, and invasion of TNBC cells.
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-TDO2, clone 7A6.1, Cat. No. MABN1537, is a highly specific mouse monoclonal antibody that targets TDO2, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TDO2 in human kidney, liver, and small intestine tissue sections.
Biological Information
ImmunogenGST-tagged recombinant human TDO2 internal fragment.
Clone7A6.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 7A6.1 targets an internal epitope of human and murine TDO2 (TDO).
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TDO2
  • TDO
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~48 kDa observed. 47.87 kDa (human) and 47.76 kDa (mouse) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected TDO2 in 10 µg of mouse liver tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN1537 04054839035326

Documentation

Anti-TDO2 Antibody, clone 7A6.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-TDO2 Antibody, clone 7A6.1 Certificates of Analysis

TitleLot Number
Anti-TDO2, clone 7A6.1 - 3862597 3862597
Anti-TDO2, clone 7A6.1 - 4090085 4090085
Anti-TDO2, clone 7A6.1 -Q2724519 Q2724519
Anti-TDO2, clone 7A6.1 Monoclonal Antibody 2909986
Anti-TDO2, clone 7A6.1 Monoclonal Antibody 2933154
Anti-TDO2, clone 7A6.1 Monoclonal Antibody 3083565
Anti-TDO2, clone 7A6.1 Monoclonal Antibody 3013238
Anti-TDO2, clone 7A6.1 Monoclonal Antibody 3156740