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MABC615 Anti-TNFα Receptor Antibody, clone 13F9.1

Anti-TNF apha Receptor Antibody, clone 13F9.1 is a highly specific mouse monoclonal antibody, that targets TNFα Receptor & has been tested in Western Blotting, and Immunohistochemistry.

Anti-TNFα Receptor Antibody, clone 13F9.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: TNFRSF1A, TNFAR, TNFR1 UniProt Number: P19438
MABC615
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC615
DescriptionAnti-TNFα Receptor Antibody, clone 13F9.1
Alternate Names
  • Tumor necrosis factor receptor superfamily member 1A
  • Tumor necrosis factor receptor 1
  • TNF-R1
  • Tumor necrosis factor receptor type I
  • TNF-RI
  • TNFR-I
  • p55
  • p60
  • CD120a
  • Tumor necrosis factor receptor superfamily member 1A, membrane form
  • Tumor necrosis factor-binding protein 1
  • TBPI
Background InformationTumor necrosis factor receptor superfamily member 1A (UniProt P19438; also known as CD120a, p55, p60, TNF-R1, TNF-RI, TNFR-I, Tumor necrosis factor-alpha receptor, Tumor necrosis factor receptor type 1) is encoded by the TNFRSF1A (also known as FPF, MS5, TNFAR, TNFAR55, TNFAR60, TNFR1) gene (Gene ID 7132) in human. It functions as a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. Upon activation, TNFalpha receptor mediates apoptotic, anti-viral, and other cellular signaling events. Binding of TNF-alpha to the extracellular domain of TNF receptor leads to homotrimerization. Proteolytic cleavage results in the relese of Tumor necrosis factor-binding protein 1 (TBP1). Defects in TNF receptor cause tumor necrosis factor receptor-associated periodic syndrome (TRAPS), a hereditary periodic fever syndrome characterized by recurrent fever, abdominal pain, localized tender skin lesions and myalgia.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-TNF apha Receptor Antibody, clone 13F9.1 is a highly specific mouse monoclonal antibody, that targets TNFα Receptor & has been tested in Western Blotting, and Immunohistochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected TNFα Receptor in 10 µg of HepG2 and RAW264 cell lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TNFα Receptor in human colon tissue and human colon cancer cells.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the extracellular domain of human TNFα Receptor.
EpitopeExtracellular domain
Clone13F9.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with spliced isoforms 1/FL-TNFR1, 2, 4/Delta6-TNFR1, and 5, but not isoform 3.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TNFRSF1A
  • TNFAR
  • TNFR1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~50/43 kDa observed. 50.5 kDa (isoform 1/FL-TNFR1 calculated), 38.7 kDa (isoform 2 calculated), 25.6 kDa (isoform 4/Delta6-TNFR1 calculated), 24.2 kDa (isoform 5 calculated).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected TNFα Receptor in 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC615 04055977171419

Documentation

Anti-TNFα Receptor Antibody, clone 13F9.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-TNFα Receptor Antibody, clone 13F9.1 Certificates of Analysis

TitleLot Number
Anti-TNFα Receptor, clone 13F9.1 - 3562044 3562044
Anti-TNFα Receptor, clone 13F9.1 - 4089937 4089937
Anti-TNFα Receptor, clone 13F9.1 -Q2573230 Q2573230

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.