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MABN769 Anti-TRPM2 Antibody, clone 9D8.1

Anti-TRPM2 Antibody, clone 9D8.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Signaling Neuroscience Gene Symbol: TRPM2, EREG1, KNP3, LTRPC2, TRPC7, NUDT9H, NUDT9L1 UniProt Number: O94759
MABN769
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWBMCulture SupernatantMonoclonal Antibody
Description
Catalogue NumberMABN769
DescriptionAnti-TRPM2 Antibody, clone 9D8.1
Alternate Names
  • Transient receptor potential cation channel subfamily M member 2
  • Estrogen-responsive element-associated gene 1 protein
  • Long transient receptor potential channel 2
  • LTrpC-2
  • LTrpC2
  • Transient receptor potential channel 7
  • TrpC7
Background InformationTransient receptor potential cation channel subfamily M member 2 (EC 3.6.1.13; UniProt O94759; also known as Estrogen-responsive element-associated gene 1 protein, Long transient receptor potential channel 2, LTrpC-2, LTrpC2, Transient receptor potential channel 7, TrpC7) is encoded by the TRPM2 (also known as EREG1, KNP3, LTRPC2, TRPC7, NUDT9H, NUDT9L1) gene (Gene ID 7226) in human. The human TRPM2 is an 1,503-amino acid, six-transmembrane protein with both N- and C-termini exposed intracellularly. TRPM2 contains a pore-forming loop domain located between the fifth and sixth transmembrane region. The N-terminal cytomplasmic region of TRPM2 contains four homologous domains with unknown function and a calmodulin-/CaM-binding IQ-like motif, which is important for channel activation. Its C-terminal cytomplasmic region contains the TRP box, a coiled-coil domain required for the assembly of TRPM2 channels, and an adenosine diphosphate ribose (ADPR) pyrophosphatase domain (Nudix-like domain or NUDT9 homology domain [NUDT9-H]). ADPR binds to the NUDT9-H domain and, in the presence of Ca2+, gates channel opening. TRPM2 activation also results in hydrolysis of ADPR into adenine monophosphate (AMP) and ribose-5-phosphate. TRPM2 is a temperature-sensitive Ca2+-permeable ion channel expressed in various tissues, including brain, lungs, liver, spleen, heart, salivary gland, and pancreas. TRPM2 is involved in redox response, cell proliferation, and insulin secretion by pancreatic β cells. TRPM2 plays a pivotal role in the death of CA1 pyramidal cells and in the development of post-ischemic brain injury. TRPM2 defect is associated with central nervous system disorders such as bipolar disorder type I, amyotrophic lateral sclerosis, Parkinson’s disease, and dementia.
References
Product Information
FormatCulture Supernatant
PresentationMouse monoclonal IgG2aκ in supernatant without preservatives.
Quality LevelMQ100
Applications
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the N-terminal cytoplasmic domain of human TRPM2.
EpitopeN-terminal cytoplasmic domain.
Clone9D8.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with all three spliced isoforms reported by UniProt (O94759).
IsotypeIgG2aκ
Species Reactivity
  • Rat
Species Reactivity NoteRat. Predicted to react with Human based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TRPM2
  • EREG1
  • KNP3
  • LTRPC2
  • TRPC7
  • NUDT9H
  • NUDT9L1
Purification MethodUnpurified
UniProt Number
Molecular Weight~170 kDa observed. 171.2 kDa (isoform 1; TRPM2-L), 165.3 kDa (isoform 2), and 95.7 kDa (isoform 3; TRPM2-S) calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat hippocampus tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TRPM2 in 10 µg of rat hippocampus tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN769 04055977311853