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MABS2240-100UL Anti-TSH Receptor Antibody, clone CS-17

Anti-TSH Receptor, clone CS-17, Cat. No. MABS2240, is a mouse monoclonal antibody that detects Thyroid-stimulating hormone receptor (TSH-R) and is tested for use in Flow Cytometry, Antagonist Function, Inhibition Assay, and Radioimmunoassay.

MABS2240-100UL
100 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABS2240-100UL
DescriptionAnti-TSH Receptor Antibody, clone CS-17
Alternate Names
  • Thyrotropin receptor
  • Thyroid-stimulating hormone receptor
  • TSH-R
Background InformationThyrotropin receptor (UniProt: P16473; also known as Thyroid-stimulating hormone receptor, TSH-R) is encoded by the TSHR (also known as LGR3) gene (Gene ID: 7253) in human. TSH-R is a multi-pass membrane protein of the G-protein coupled receptor (GPCR) family that is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to generate the mature form that contains four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. The extracellular domain is linked to a distal signal-specific domain the hinge region that is attached to a transmembrane domain consisting of extracellular and intracellular loops. It serves as a receptor the thyroid-stimulating hormone and TSH binding to this receptor induces thyrocyte growth and proliferation primarily by activating the adenylate cyclase signaling pathway. Clone CS-17 acts allosterically and serves as an inverse agonist for TSH-R constitutive activity. When administered in vivo it is shown to suppress thyroid function and reduces serum T4 levels in BALB/c mice. (Ref.: Chen, RR., et al. (2018). Thyroid. 28(77); 933-940; Chen, CR., et al. (2007). Endocrinology. 148(5); 2375-2382).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-TSH Receptor, clone CS-17, Cat. No. MABS2240, is a mouse monoclonal antibody that detects Thyroid-stimulating hormone receptor (TSH-R) and is tested for use in Flow Cytometry, Antagonist Function, Inhibition Assay, and Radioimmunoassay.
Key Applications
  • Flow Cytometry
  • Inhibition
  • Radioimmunoassay
  • Agonist or Inhibitor
Application NotesTested Applications

Flow Cytometry: A representative lot detected TSH Receptor in Flow Cytometry applications (Chen, C.R., et al. (2007). Endocrinology. 148(5):2375-82; Chen, C.R., et al. (2009). Endocrinology. 150(7):3401-8; Chen, C.R., et al. (2018). Thyroid. 28(7):933-940).

Inhibition: A representative lot inhibited the constitutive activity of the TSH Receptor. (Furmaniak, J. et al. (2012). Autoimmun Highlights. 4(1):11-26).

Radioimmunoassay: A representative lot detected TSH Receptor in Radioimmunoassay applications (Chen, C.R., et al. (2007). Endocrinology. 148(5):2375-82).

Antagonist Function: A representative lot bloced TSH binding to the TSH Receptor. Endocrinology. 150(7):3401-8; Chen, C.R., et al. (2018). Thyroid. 28(7):933-940).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenAdenovirus expressing TSH receptor fragment (aa 22-289). adenovirus.
EpitopeN-terminal
CloneCS-17
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone CS-17 is a mouse monoclonal antibody that detects human Thyrotropin receptor. It targets an epitope within the first extracellular domain from the N-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TSHR
Purification MethodProtein G purified
UniProt Number
Molecular Weight86.83 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in TSH-R transfected HEK293 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected TSH Receptor in one million TSH-R transfected HEK293 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2240-100UL 04065267216669

Documentation

Anti-TSH Receptor Antibody, clone CS-17 Certificates of Analysis

TitleLot Number
Anti-TSH Receptor, clone CS-17 - 3993578 3993578
Anti-TSH Receptor, clone CS-17 - 3994712 3994712
Anti-TSH Receptor, clone CS-17 - 4000743 4000743
Anti-TSH Receptor, clone CS-17 - 4004385 4004385
Anti-TSH Receptor, clone CS-17 - 4009767 4009767
Anti-TSH Receptor, clone CS-17 - 4010106 4010106
Anti-TSH Receptor, clone CS-17 - 4012164 4012164
Anti-TSH Receptor, clone CS-17 - 4013918 4013918
Anti-TSH Receptor, clone CS-17 - 4062767 4062767
Anti-TSH Receptor, clone CS-17 - 4080521 4080521
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