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MABC1205 Anti-UDP-Galactose Transporter Antibody, clone 1H10.1

Detect UDP-galactose transporter (SLC35A2) using this mouse monoclonal Anti-UDP-Galactose Transporter, clone 1H10.1, Cat. No. MABC1205, validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

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Research Category: Apoptosis & Cancer Gene Symbol: SLC35A2, CDG2M, UGALT, UGT, UGTL UniProt Number: P78381
MABC1205
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1205
DescriptionAnti-UDP-Galactose Transporter Antibody, clone 1H10.1
Alternate Names
  • UDP-galactose translocator
  • Solute carrier family 35 member A2
  • UDP-Gal-Tr
  • UDP-galactose transporter
  • UDP-galactose transporter
  • UGT
Background InformationUDP-galactose translocator (UniProt P78381; also known as Solute carrier family 35 member A2, UDP-Gal-Tr, UDP-galactose transporter, UDP-galactose transporter, UGT) is encoded by the SLC35A2 (also known as CDG2M, UGALT, UGT, UGTL) gene (Gene ID 7355) in human. UGT is a multi-pass membrane protein that transports UDP-galactose from the cytosol into the Golgi vesicles where it serves as a glycosyl donor for the generation of glycans. Its short N-terminal region, composed of 35 amino-acid residues, is reported to be crucial for galactosylation of N-glycans. Mutations in SLC35A2 gene are known to reduce UDP-galactose transport that leads to galactose-deficient glycoproteins resulting in developmental delays, hyotonia, ocular anomalies, and brain malfunctions. Higher expression of UGT mRNA has been reported in human colon cancer. Ref.: Ng, BG., et al. (2013). Am. J. Human Gen. 92, 632-636.
References
Product Information
FormatPurified
PresentationPurified mouse IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect UDP-galactose transporter (SLC35A2) using this mouse monoclonal Anti-UDP-Galactose Transporter, clone 1H10.1, Cat. No. MABC1205, validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunocytochemistry Analysis: Clone 1H10.1 hybridoma culture supernatant immunostained Golgi apparatus in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized A431 cells.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected UDP-Gal transporter in human colon, liver, lung, and placenta tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human UDP-Gal transporter C-terminal end sequence.
Clone1H10.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityThe immunogen sequence is 100% present in spliced isoforms 1 (UGT2) and 3, while being only partially present in spliced isoforms 2 (UGT1), 4, and 5 reported by UniProt (P78381), reactivity toward these latter isoforms has not been confirmed.
IsotypeIgG3κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SLC35A2
  • CDG2M
  • UGALT
  • UGT
  • UGTL
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~50 kDa observed. 14.31/25.18 k.Da (isoform 1/3) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human placenta tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UDP-Gal Transporter in 10 µg of human placenta tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1205 04054839091971